ArticleScientific reports2015
Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Botulinum neurotoxin: Tracking the transition from lethal dose toNAM journal · 2025Article
- Neural organoids as advanced tools for neurotoxicity modeling.Current research in toxicology · 2025Review
- Three-dimensional bioprinting of stem cell-derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function.Biotechnology and bioengineering · 2023Article
- The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration.International journal of molecular sciences · 2023Article
- Recent Developments in Botulinum Neurotoxins Detection.Microorganisms · 2022Review
- Emerging Opportunities in Human Pluripotent Stem-Cells Based Assays to Explore the Diversity of Botulinum Neurotoxins as Future Therapeutics.International journal of molecular sciences · 2021Review
- hiPSC-Derived Neurons Provide a Robust and Physiologically RelevantFrontiers in pharmacology · 2020Article
- Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations.FEBS letters · 2019Article
- iPSC-Derived Brain Endothelium Exhibits Stable, Long-Term Barrier Function in Perfused Hydrogel Scaffolds.Stem cell reports · 2019Article
- Development of a Novel FIJI-Based Method to Investigate Neuronal Circuitry in Neonatal Mice.Developmental neurobiology · 2018Article
- Why the impact of mechanical stimuli on stem cells remains a challenge.Cellular and molecular life sciences : CMLS · 2018Review
- Designing well-defined photopolymerized synthetic matrices for three-dimensional culture and differentiation of induced pluripotent stem cells.Biomaterials science · 2018Article
- Assessment of ELISA as endpoint in neuronal cell-based assay for BoNT detection using hiPSC derived neurons.Journal of pharmacological and toxicological methods · 2017Article
- Uniform neural tissue models produced on synthetic hydrogels using standard culture techniques.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Modeling Neurovascular Disorders and Therapeutic Outcomes with Human-Induced Pluripotent Stem Cells.Frontiers in bioengineering and biotechnology · 2017Review
- 3D culture models of Alzheimer's disease: a road map to a "cure-in-a-dish".Molecular neurodegeneration · 2016Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Botulinum neurotoxin (BoNT) detection provides a useful model for validating cell-based neurotoxicity screening approaches, as sensitivity is dependent on functionally competent neurons and clear quantitative endpoints are available for correlating results to approved animal testing protocols. Here, human induced pluripotent stem cell (iPSC)-derived neuronal cells were cultured on chemically-defined poly(ethylene glycol) (PEG) hydrogels formed by "thiol-ene" photopolymerization and tested as a cell-based neurotoxicity assay by determining sensitivity to active BoNT/A1. BoNT/A1 sensitivity was comparable to the approved in vivo mouse bioassay for human iPSC-derived neurons and neural stem cells (iPSC-NSCs) cultured on PEG hydrogels or treated tissue culture polystyrene (TCP) surfaces. However, maximum sensitivity for BoNT detection was achieved two weeks earlier for iPSC-NSCs that were differentiated and matured on PEG hydrogels compared to TCP. Therefore, chemically-defined synthetic hydrogels offer benefits over standard platforms when optimizing culture conditions for cell-based screening and achieve sensitivities comparable to an approved animal testing protocol.
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Registered trials
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