ArticleRapid communications in mass spectrometry : RCM2015
Effects of demineralization on the stable isotope analysis of bone samples.
Article in Rapid communications in mass spectrometry : RCM, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Beyond trophic morphology: stable isotopes reveal ubiquitous versatility in marine turtle trophic ecology.Biological reviews of the Cambridge Philosophical Society · 2019Pooled it
- Green Sea Turtle Recruitment in the Eastern North Pacific: Patterns Identified Using Geochemical Signatures in Bones.Ecology and evolution · 2026Article
- Ecological Niche Changes of Two Sympatric Species (Ecology and evolution · 2025Article
- Geographical traceability of gelatin in China using stable isotope ratio analysis.Frontiers in nutrition · 2023Article
- Dietary shifts may underpin the recovery of a large carnivore population.Biology letters · 2022Article
- Article
- Proteome Variation with Collagen Yield in Ancient Bone.Journal of proteome research · 2021Article
- Stable isotope records of sei whale baleens from Chilean Patagonia as archives for feeding and migration behavior.Ecology and evolution · 2020Article
- Stable isotope discrimination factors and between-tissue isotope comparisons for bone and skin from captive and wild green sea turtles (Chelonia mydas).Rapid communications in mass spectrometry : RCM · 2017Article
- Intrapopulation variability in the timing of ontogenetic habitat shifts in sea turtles revealed using δThe Journal of animal ecology · 2017Article
- Growth dynamics of juvenile loggerhead sea turtles undergoing an ontogenetic habitat shift.Oecologia · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
rationaleThe sampling of sequential, annually formed bone growth layers for stable carbon (δ(13)C values) and nitrogen (δ(15)N values) isotope analysis (SIA) can provide a time series of foraging ecology data. To date, no standard protocol exists for the pre-SIA treatment of cortical samples taken from fresh, modern, bones.
methodsBased on the SIA of historical bone, it is assumed that fresh bone samples must be pre-treated with acid prior to SIA. Using an elemental analyzer coupled to an isotope ratio mass spectrometer to measure stable carbon and nitrogen ratios, we tested the need to acidify cortical bone powder with 0.25 M HCl prior to SIA to isolate bone collagen for the determination of δ(13)C and δ(15)N values. We also examined the need for lipid extraction to remove potential biases related to δ(13)C analysis, based on a C:N ratio threshold of 3.5.
resultsIt was found that acidification of micromilled cortical bone samples from marine turtles does not affect their δ(15)N values, and the small effect acidification has on δ(13)C values can be mathematically corrected for, thus eliminating the need for pre-SIA acidification of cortical bone. The lipid content of the cortical bone samples was low, as measured by their C:N ratios, indicating that lipid extracting cortical bone samples from modern marine turtles is unnecessary.
conclusionsWe present a standard protocol for testing fresh, modern cortical bone samples prior to SIA, facilitating direct comparison of future studies. Based on the results obtained from marine turtle bones, pre-acidification and lipid removal of cortical bone are not recommended. This is especially useful as there is frequently not enough bone material removed via micromilling of sequential growth layers to accommodate both acid treatment and SIA.
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