ArticleJournal of assisted reproduction and genetics2015
A single-cell assay for telomere DNA content shows increasing telomere length heterogeneity, as well as increasing mean telomere length in human spermatozoa with advancing age.
Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 64 citations in OpenAlex.
- Telomere Length, a New Biomarker of Male (in)Fertility? A Systematic Review of the Literature.Genes · 2023Pooled it
- The association of female and male infertility with telomere length (Review).International journal of molecular medicine · 2019Pooled it
- Beyond semen analysis: in men with normal semen parameters telomere attrition and oxidative imbalance distinguish those fertile from those with infertility.Journal of translational medicine · 2026Article
- Telomere Length Abnormality: Investigating Approaches and Correlations with Cancer, Bone Marrow Failure and Hematological Malignancies.Biomedicines · 2025Review
- Paternal effects on telomere integrity during the sperm-to-embryo transition.Current opinion in genetics & development · 2025Review
- Article
- Techniques for assessing telomere length: A methodological review.Computational and structural biotechnology journal · 2024Review
- The Notable Role of Telomere Length Maintenance in Complex Diseases.Biomedicines · 2024Review
- Microfluidics-A novel technique for high-quality sperm selection for greater ART outcomes.FASEB bioAdvances · 2024Review
- Telomeres and SIRT1 as Biomarkers of Gamete Oxidative Stress, Fertility, and Potential IVF Outcome.International journal of molecular sciences · 2024Review
- Evaluation of telomere length and telomerase activity on predicting in vitro fertilization treatment outcomes.Journal of assisted reproduction and genetics · 2024Review
- The crosstalk between telomeres and DNA repair mechanisms: an overview to mammalian somatic cells, germ cells, and preimplantation embryos.Journal of assisted reproduction and genetics · 2024Review
- Telomere Elongation During Pre-Implantation Embryo Development.Advances in anatomy, embryology, and cell biology · 2024Review
- Emergence and influence of sequence bias in evolutionarily malleable, mammalian tandem arrays.BMC biology · 2023Article
- Transposon insertion profiling by sequencing (TIPseq) identifies novel LINE-1 insertions in human sperm.Journal of assisted reproduction and genetics · 2023Article
- A pilot study of LINE-1 copy number and telomere length with aging in human sperm.Journal of assisted reproduction and genetics · 2023Article
- Telomere Length in Human Spermatogenic Cells as a New Potential Predictor of Clinical Outcomes in ART Treatment with Intracytoplasmic Injection of Testicular Spermatozoa.International journal of molecular sciences · 2023Article
- Article
- Insights into the Role of Telomeres in Human Embryological Parameters. Opinions Regarding IVF.Journal of developmental biology · 2021Review
- Telomere Length in Metaphase Chromosomes of Human Triploid Zygotes.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeThe effect of age on telomere length heterogeneity in men has not been studied previously. Our aims were to determine the relationship between variation in sperm telomere length (STL), men's age, and semen parameters in spermatozoa from men undergoing in vitro fertilization (IVF) treatment.
methodsTo perform this prospective cross-sectional pilot study, telomere length was estimated in 200 individual spermatozoa from men undergoing IVF treatment at the NYU Fertility Center. A novel single-cell telomere content assay (SCT-pqPCR) measured telomere length in individual spermatozoa.
resultsTelomere length among individual spermatozoa within an ejaculate varies markedly and increases with age. Older men not only have longer STL but also have more variable STL compared to younger men. STL from samples with normal semen parameters was significantly longer than that from samples with abnormal parameters, but STL did not differ between spermatozoa with normal versus abnormal morphology.
conclusionThe marked increase in STL heterogeneity as men age is consistent with a role for ALT during spermatogenesis. No data have yet reported the effect of age on STL heterogeneity. Based on these results, future studies should expand this modest sample size to search for molecular evidence of ALT in human testes during spermatogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.