ArticleBiomaterials2015
Engineering vascularized soft tissue flaps in an animal model using human adipose-derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle.
Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 79 citations in OpenAlex.
- Silk fibroin/nano-hydroxyapatite scaffolds with graphene oxide induce macrophage M2 polarization and enhance angiogenesis to facilitate bone repair.RSC advances · 2026Article
- Regenerative Approach for Improving Flap Survival: Perspective of Angiogenesis.Biomimetics (Basel, Switzerland) · 2026Review
- Autonomization of Microvascular Free Flaps in Reconstructive Surgery: A Narrative Review.Microsurgery · 2026Review
- Current Modalities in Soft-Tissue Reconstruction and Vascularized Adipose Engineering.Biomolecules · 2025Review
- Adipose-derived mesenchymal stem cell-incorporated PLLA porous microspheres for cartilage regeneration.Animal models and experimental medicine · 2024Article
- Autologous fat grafting for postoperative breast reconstruction: A systemic review.Regenerative therapy · 2024Review
- Harnessing the synergy of perfusable muscle flap matrix and adipose-derived stem cells for prevascularization and macrophage polarization to reconstruct volumetric muscle loss.Bioactive materials · 2023Article
- Acellular Tissue-Engineered Vascular Grafts from Polymers: Methods, Achievements, Characterization, and Challenges.Polymers · 2022Review
- Techniques and Innovations in Flap Engineering: A Review.Plastic and reconstructive surgery. Global open · 2022Article
- Adipogenesis or osteogenesis: destiny decision made by mechanical properties of biomaterials.RSC advances · 2022Review
- Full-Thickness Oral Mucoperiosteal Defects: Challenges and Opportunities.Tissue engineering. Part B, Reviews · 2022Review
- Research Progress on the Immunogenicity and Regeneration of Acellular Adipose Matrix: A Mini Review.Frontiers in bioengineering and biotechnology · 2022Review
- Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles.International journal of molecular sciences · 2021Article
- [Preliminary exploration on the application of hydrogel from acellular porcine adipose tissue to assist lipofilling].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Article
- [Early effect of graphene oxide-carboxymethyl chitosan hydrogel loaded with interleukin 4 and bone morphogenetic protein 2 on bone immunity and repair].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Article
- A biocompatible vascularized graphene oxide (GO)-collagen chamber with osteoinductive and anti-fibrosis effects promotes bone regenerationTheranostics · 2020Article
- Application of Chitosan in Bone and Dental Engineering.Molecules (Basel, Switzerland) · 2019Review
- Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation.Materials (Basel, Switzerland) · 2019Review
- Tissue Engineering of the Microvasculature.Comprehensive Physiology · 2019Review
- Silicon-Enhanced Adipogenesis and Angiogenesis for Vascularized Adipose Tissue Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Insufficient neovascularization is associated with high levels of resorption and necrosis in autologous and engineered fat grafts. We tested the hypothesis that incorporating angiogenic growth factor into a scaffold-stem cell construct and implanting this construct around a vascular pedicle improves neovascularization and adipogenesis for engineering soft tissue flaps. Poly(lactic-co-glycolic-acid/polyethylene glycol (PLGA/PEG) microspheres containing vascular endothelial growth factor (VEGF) were impregnated into collagen-chitosan scaffolds seeded with human adipose-derived stem cells (hASCs). This setup was analyzed in vitro and then implanted into isolated chambers around a discrete vascular pedicle in nude rats. Engineered tissue samples within the chambers were harvested and analyzed for differences in vascularization and adipose tissue growth. In vitro testing showed that the collagen-chitosan scaffold provided a supportive environment for hASC integration and proliferation. PLGA/PEG microspheres with slow-release VEGF had no negative effect on cell survival in collagen-chitosan scaffolds. In vivo, the system resulted in a statistically significant increase in neovascularization that in turn led to a significant increase in adipose tissue persistence after 8 weeks versus control constructs. These data indicate that our model-hASCs integrated with a collagen-chitosan scaffold incorporated with VEGF-containing PLGA/PEG microspheres supported by a predominant vascular vessel inside a chamber-provides a promising, clinically translatable platform for engineering vascularized soft tissue flap. The engineered adipose tissue with a vascular pedicle could conceivably be transferred as a vascularized soft tissue pedicle flap or free flap to a recipient site for the repair of soft-tissue defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.