Simultaneous two color image capture for sub-diffraction localization fluorescence microscopy.

Ben J Glasgow, Lie Ma

Abstract read

In one paragraph

Article in Micron (Oxford, England : 1993), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami.Proceedings of SPIE--the International Society for Optical Engineering · 2016
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ben J GlasgowDepartments of Ophthalmology, Pathology and Laboratory Medicine, Jules Stein Eye Institute, University of California, Los Angeles, 100 Stein Plaza Rm. BH 623, Los Angeles, CA 90095, USA. Electronic address: bglasgow@mednet.ucla.edu.

Lie MaDepartments of Ophthalmology, Pathology and Laboratory Medicine, Jules Stein Eye Institute, University of California, Los Angeles, 100 Stein Plaza Rm. BH 623, Los Angeles, CA 90095, USA.

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gregory Darin Field, DAVID S WILLIAMS · 1985 to 2026

$16.8M

Proteins in the Molecular Mechanisms of Tear Film FormationR01EY011224 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GLASGOW, BEN J · 2001 to 2020

$6.8M

PROTEINS IN MOLECULAR MECHANISMS OF TEAR FILM FORMATIONR29EY011224 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GLASGOW, BEN J · 1996 to 2000

$116k

NEI NIH HHS EY00331NEI NIH HHS EY11224NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY011224NEI NIH HHS R29 EY011224

6 · The paper itself

Abstract

A sub-diffraction limit fluorescence localization microscope was constructed using a standard cooled 1.4 mega-pixel fluorescence charge-coupled device (CCD) camera to simultaneously resolve closely adjacent paired quantum dots on a flat surface with emissions of 540 and 630 nm. The images of the overlapping Airy discs were analyzed to determine the center of the point spread function after noise reduction using Fourier transformation analysis. The Cartesian coordinates of the centers of the point spread functions were compared in serial images. Histograms constructed from serial images fit well to Gaussian functions for resolving two quantum dots separated by as little as 10nm in the x-y coordinates. Statistical analysis of multiple pairs validated discrimination of inter-fluorophore distances that vary by 10nm. The method is simple and developed for x-y resolution of dilute fluorophores on a flat surface, not serial z sectioning.

Indexed as

ColorImage Processing, Computer-AssistedMicroscopy, FluorescenceOptical ImagingCCD cameraDiffraction unlimitedFluorescenceLocalization microscopyQuantum dotsSuper-resolution microscopy

Identifiers

PMID26409111

DOI10.1016/j.micron.2015.09.007

PMCPMC4674362

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.