Evidence map›Paper›PMID 26407890›Full record

ArticleApplied and environmental microbiology2015

A virulent phage infecting Lactococcus garvieae, with homology to Lactococcus lactis phages.

Giovanni Eraclio, Denise M Tremblay, Alexia Lacelle-Côté, Simon J Labrie, Maria Grazia Fortina, Sylvain Moineau

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. A Plasmid-Encoded Surface Polysaccharide Partly BlocksInternational journal of molecular sciences · 2025
    Article
  4. Review
  5. Article
  6. Phage Therapy as a Focused Management Strategy in Aquaculture.International journal of molecular sciences · 2021
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Giovanni EraclioDepartment of Food, Environmental and Nutritional Sciences, Division of Food Microbiology and Bioprocesses, University of Milan, Milan, Italy.
Denise M TremblayFélix d'Hérelle Reference Center for Bacterial Viruses & GREB, Faculté de Médecine Dentaire, Université Laval, Québec City, Québec, Canada.
Alexia Lacelle-CôtéFélix d'Hérelle Reference Center for Bacterial Viruses & GREB, Faculté de Médecine Dentaire, Université Laval, Québec City, Québec, Canada Département de biochimie, de microbiologie et de bio-informatique & PROTEO, Faculté des sciences et de génie, Université Laval, Québec City, Québec, Canada.
Simon J LabrieFélix d'Hérelle Reference Center for Bacterial Viruses & GREB, Faculté de Médecine Dentaire, Université Laval, Québec City, Québec, Canada Département de biochimie, de microbiologie et de bio-informatique & PROTEO, Faculté des sciences et de génie, Université Laval, Québec City, Québec, Canada.
Maria Grazia FortinaDepartment of Food, Environmental and Nutritional Sciences, Division of Food Microbiology and Bioprocesses, University of Milan, Milan, Italy.
Sylvain MoineauFélix d'Hérelle Reference Center for Bacterial Viruses & GREB, Faculté de Médecine Dentaire, Université Laval, Québec City, Québec, Canada Département de biochimie, de microbiologie et de bio-informatique & PROTEO, Faculté des sciences et de génie, Université Laval, Québec City, Québec, Canada Sylvain.Moineau@bcm.ulaval.ca.
Université Laval · CAUniversity of Milan · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only two L. garvieae strains out of 73 tested. Phage GE1 has a double-stranded DNA genome of 24,847 bp containing 48 predicted open reading frames (ORFs). Putative functions could be assigned to only 14 ORFs, and significant matches in public databases were found for only 17 ORFs, indicating that GE1 is a novel phage and its genome contains several new viral genes and encodes several new viral proteins. Of these 17 ORFs, 16 were homologous to deduced proteins of virulent phages infecting the dairy bacterium Lactococcus lactis, including previously characterized prolate-headed phages. Comparative genome analysis confirmed the relatedness of L. garvieae phage GE1 to L. lactis phages c2 (22,172 bp) and Q54 (26,537 bp), although its genome organization was closer to that of phage c2. Phage GE1 did not infect any of the 58 L. lactis strains tested. This study suggests that phages infecting different lactococcal species may have a common ancestor.

Indexed as

Base SequenceCapsidCapsid ProteinsDNA, ViralGenes, ViralGenome, ViralHost SpecificityLactococcus lactisMicroscopy, ElectronMolecular Sequence DataOpen Reading FramesProteomeSequence Analysis, DNASiphoviridaeSoil MicrobiologyVirionCapsid ProteinsDNA, ViralProteome

Identifiers

PMID26407890
PMCPMC4644644
OpenAlexW2181166417

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.