ArticlePloS one2015
Refining DNA Barcoding Coupled High Resolution Melting for Discrimination of 12 Closely Related Croton Species.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Secondary Metabolites fromMetabolites · 2025Review
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Decoding herbal materials of TCM preparations with the multi-barcode sequencing approach.Scientific reports · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Species Quantification in Complex Herbal Formulas-Vector Control Quantitative Analysis as a New Method.Frontiers in pharmacology · 2020Article
- Article
- Multiuse of Bar-HRM for Ophiocordyceps sinensis identification and authentication.Scientific reports · 2018Article
- Bar-HRM for authenticating soursop (Annona muricata) tea.Scientific reports · 2018Article
- Authenticity Testing and Detection ofGenes · 2018Article
- Species identification approach for both raw materials and end products of herbal supplements from Tinospora species.BMC complementary and alternative medicine · 2018Article
- Should DNA sequence be incorporated with other taxonomical data for routine identifying of plant species?BMC complementary and alternative medicine · 2017Article
- Combination of DNA isolation and RP-HPLC analysis method for bark samples of Saraca asoca and its adulterant.3 Biotech · 2017Article
- Rapidly discriminate commercial medicinal Pulsatilla chinensis (Bge.) Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay.Scientific reports · 2017Article
- Evaluation of a DNA-based method for spice/herb authentication, so you do not have to worry about what is in your curry, buon appetito!PloS one · 2017Article
- Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products.Evidence-based complementary and alternative medicine : eCAM · 2017Review
- The Potential Power of Bar-HRM Technology in Herbal Medicine Identification.Frontiers in plant science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA barcoding coupled high resolution melting (Bar-HRM) is an emerging method for species discrimination based on DNA dissociation kinetics. The aim of this work was to evaluate the suitability of different primer sets, derived from selected DNA regions, for Bar-HRM analysis of species in Croton (Euphorbiaceae), one of the largest genera of plants with over 1,200 species. Seven primer pairs were evaluated (matK, rbcL1, rbcL2, rbcL3, rpoC, trnL and ITS1) from four plastid regions, matK, rbcL, rpoC, and trnL, and the nuclear ribosomal marker ITS1. The primer pair derived from the ITS1 region was the single most effective region for the identification of the tested species, whereas the rbcL1 primer pair gave the lowest resolution. It was observed that the ITS1 barcode was the most useful DNA barcoding region overall for species discrimination out of all of the regions and primers assessed. Our Bar-HRM results here also provide further support for the hypothesis that both sequence and base composition affect DNA duplex stability.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.