Evidence map›Paper›PMID 26406460›Full record

ArticleBiosensors & bioelectronics2016

3D-printed supercapacitor-powered electrochemiluminescent protein immunoarray.

Karteek Kadimisetty, Islam M Mosa, Spundana Malla, Jennifer E Satterwhite-Warden, Tyler M Kuhns, Ronaldo C Faria, Norman H Lee, James F Rusling

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
8.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 171 citations in OpenAlex.

  1. Review
  2. Review
  3. Nanobiosensing Based on Electro-Optically Modulated Technology.Nanomaterials (Basel, Switzerland) · 2023
    Review
  4. Organ Chips and Visualization of Biological Systems.Advances in experimental medicine and biology · 2023
    Article
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  17. Article
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  20. 3D-Printed Immunosensor Arrays for Cancer Diagnostics.Sensors (Basel, Switzerland) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 4 countries.

Karteek KadimisettyDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Islam M MosaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA; Department of Chemistry, Tanta University, Tanta 31527, Egypt.
Spundana MallaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Jennifer E Satterwhite-WardenDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Tyler M KuhnsDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Ronaldo C FariaDepartamento de Química, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
Norman H LeeDepartment of Pharmacology & Physiology, George Washington University, Washington, DC, USA.
James F RuslingDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA; Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032, USA; School of Chemistry, National University of Ireland, Galway, Ireland; Institute of Materials Science, University of Connecticut, Storrs, CT 06269, USA. Electronic address: james.rusling@uconn.edu.
University of Connecticut · USGeorge Washington University · USUniversidade Federal de São Carlos · BR

Funding

Protein Biomarker Arrays for Personalized Treatment of Prostate CancerR01EB016707 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2014 to 2017
$1.4M
Protein Biosensor Arrays Based on NanomaterialsR01EB014586 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2011 to 2014
$1.4M
NIBIB NIH HHS EB014586NIBIB NIH HHS EB016707NIBIB NIH HHS R01 EB014586NIBIB NIH HHS R01 EB016707
6 · The paper itself

Abstract

Herein we report a low cost, sensitive, supercapacitor-powered electrochemiluminescent (ECL) protein immunoarray fabricated by an inexpensive 3-dimensional (3D) printer. The immunosensor detects three cancer biomarker proteins in serum within 35 min. The 3D-printed device employs hand screen printed carbon sensors with gravity flow for sample/reagent delivery and washing. Prostate cancer biomarker proteins, prostate specific antigen (PSA), prostate specific membrane antigen (PSMA) and platelet factor-4 (PF-4) in serum were captured on the antibody-coated carbon sensors followed by delivery of detection-antibody-coated Ru(bpy)3(2+) (RuBPY)-doped silica nanoparticles in a sandwich immunoassay. ECL light was initiated from RuBPY in the silica nanoparticles by electrochemical oxidation with tripropylamine (TPrA) co-reactant using supercapacitor power and ECL was captured with a CCD camera. The supercapacitor was rapidly photo-recharged between assays using an inexpensive solar cell. Detection limits were 300-500f gmL(-1) for the 3 proteins in undiluted calf serum. Assays of 6 prostate cancer patient serum samples gave good correlation with conventional single protein ELISAs. This technology could provide sensitive onsite cancer diagnostic tests in resource-limited settings with the need for only moderate-level training.

Indexed as

Electric Power SuppliesPrinting, Three-DimensionalConductometryElectric CapacitanceEquipment DesignEquipment Failure AnalysisImmunoassayLuminescent MeasurementsMiniaturizationProtein Array AnalysisReproducibility of ResultsSensitivity and Specificity3D-printingBiomarker proteinsECL immunoarrayMicrofluidicsProstate cancerSupercapacitor

Identifiers

PMID26406460
PMCPMC4673015
OpenAlexW1963428971

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.