ArticlePloS one2015
Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers, 1 of them a synthesis that pooled it.
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Who cites it
133 citing papers in PubMed, 1 synthesis or guideline pooled it, 314 citations in OpenAlex.
- Reviewing bryophyte-microorganism association: insights into environmental optimization.Frontiers in microbiology · 2024Pooled it
- ABCG transporters are involved in the accumulation of specialized metabolites in the oil bodies of Marchantia polymorpha.Plant & cell physiology · 2026Article
- Engineering Auronidin Production in Marchantia polymorpha Enables a New Class of Plant-Derived Textile Pigments.ACS synthetic biology · 2026Article
- Rapid plastid isolation reveals the chloroplast proteome and structures of the chlororibosome large subunit and RuBisCO inbioRxiv : the preprint server for biology · 2026Article
- Structure determination and dual targeting of a plant TACO1 identifies its ancient role as an organelle translation regulator.bioRxiv : the preprint server for biology · 2026Article
- Initiation of asexual reproduction by the AP2/ERF gene GEMMIFER in Marchantia polymorpha.Current biology : CB · 2026Article
- Spatial organization of plant defense at the infection front.bioRxiv : the preprint server for biology · 2026Article
- Diversification of functional requirements for proteolysis of auxin response factors.Nature communications · 2026Article
- The SPL-family transcription factor MpSPL3 orchestrates the proper regulation of vegetative and reproductive programs in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2026Article
- Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha.Journal of experimental botany · 2025Article
- Cationic amino acid transporters (CAT) enhance accumulation and susceptibility to the systemic herbicide L-phosphinothricin.Nature communications · 2025Article
- The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha.Journal of plant research · 2025Article
- Evolutionary Refinement of Mitochondrial and Plastid Targeting Sequences Coincides with the Late Diversification of Land Plants.Molecular biology and evolution · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- SHOT GLASS, an R2R3-MYB transcription factor, promotes gemma cup and gametangiophore development in Marchantia polymorpha.The New phytologist · 2025Article
- MpmiR319 promotes gemma/gemma cup formation in the liverwort Marchantia polymorpha.Journal of experimental botany · 2025Article
- Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.Scientific reports · 2025Article
- Evolutionary-conserved RLF, a cytochrome bThe New phytologist · 2025Article
- Shikimate Kinase-Like 1 Participates in an Ancient and Conserved Role Contributing to Chloroplast Biogenesis in Land Plants.Molecular biology and evolution · 2025Article
- Involvement of a NIMA-related kinase in cell division of the liverwort Marchantia polymorpha.Plant & cell physiology · 2025Article
73 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.
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