ArticleeLife2015
A mitotic kinase scaffold depleted in testicular seminomas impacts spindle orientation in germ line stem cells.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 51 citations in OpenAlex.
- AKAP2 is required for assembly of cytoskeletal signaling complexes that promote growth and metastasis of triple-negative breast cancer.The Journal of biological chemistry · 2026Article
- Article
- The cAMP-PKA signaling initiates mitosis by phosphorylating Bora.Nature communications · 2025Article
- Protein kinase A and local signaling in cancer.The Biochemical journal · 2024Review
- LGN loss randomizes spindle orientation and accelerates tumorigenesis in PTEN-deficient epidermis.Molecular biology of the cell · 2024Article
- The miR-669a-5p/G3BP/HDAC6/AKAP12 Axis Regulates Primary Cilia Length.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Centrosome linker protein C-Nap1 maintains stem cells in mouse testes.EMBO reports · 2022Article
- Spindle positioning and its impact on vertebrate tissue architecture and cell fate.Nature reviews. Molecular cell biology · 2021Review
- Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (Animals : an open access journal from MDPI · 2021Article
- Kinase-anchoring proteins in ciliary signal transduction.The Biochemical journal · 2021Review
- AKAP Signaling Islands: Venues for Precision Pharmacology.Trends in pharmacological sciences · 2020Review
- Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles.The Journal of biological chemistry · 2020Article
- Asymmetric cell division and replicative aging: a new perspective from the spindle poles.Current genetics · 2020Review
- PP2A--B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA.Journal of cell science · 2020Article
- Notch signaling regulates Akap12 expression and primary cilia length during renal tubule morphogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- The Role of APAL/ST8SIA6-AS1 lncRNA in PLK1 Activation and Mitotic Catastrophe of Tumor Cells.Journal of the National Cancer Institute · 2020Article
- Mitotic kinase anchoring proteins: the navigators of cell division.Cell cycle (Georgetown, Tex.) · 2020Review
- Article
- NDP52 tunes cortical actin interaction with astral microtubules for accurate spindle orientation.Cell research · 2019Article
- Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes.Molecular biology of the cell · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Correct orientation of the mitotic spindle in stem cells underlies organogenesis. Spindle abnormalities correlate with cancer progression in germ line-derived tumors. We discover a macromolecular complex between the scaffolding protein Gravin/AKAP12 and the mitotic kinases, Aurora A and Plk1, that is down regulated in human seminoma. Depletion of Gravin correlates with an increased mitotic index and disorganization of seminiferous tubules. Biochemical, super-resolution imaging, and enzymology approaches establish that this Gravin scaffold accumulates at the mother spindle pole during metaphase. Manipulating elements of the Gravin-Aurora A-Plk1 axis prompts mitotic delay and prevents appropriate assembly of astral microtubules to promote spindle misorientation. These pathological responses are conserved in seminiferous tubules from Gravin(-/-) mice where an overabundance of Oct3/4 positive germ line stem cells displays randomized orientation of mitotic spindles. Thus, we propose that Gravin-mediated recruitment of Aurora A and Plk1 to the mother (oldest) spindle pole contributes to the fidelity of symmetric cell division.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.