ArticleThe ISME journal2016
Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 329 papers.
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Who cites it
329 citing papers in PubMed, 677 citations in OpenAlex.
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- Comparative Shotgun Metagenomics of Microbial Communities Associated with Two Geothermal Systems in Kazakhstan.Biology · 2026Article
- Electrochemical characterization of C. pasteurianum hydrogenase II and three site-directed variants: catalysis and inhibition.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Genetic dissection of formate hydrogenlyase-2 function: a metalloenzyme involved in bacterial hydrogen production.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Trace gas oxidation supports sub-surface microbial communities across Namib Desert fog and aridity gradients.Applied and environmental microbiology · 2026Article
- Phylogenetic reconciliation supports a methanogenic ancestor of the Archaea and a derived origin for host-associated lineages.Nature communications · 2026Article
- Enrichment of Hydrogen-Oxidizing Bacteria Using a Hybrid Biological-Inorganic System Under a High COMicroorganisms · 2026Article
- Adaptations in electron transport chain complexes of clinical Rhodococcus equi revealed through comparative genomics.World journal of microbiology & biotechnology · 2026Article
- QM/MM Modeling of the Electronic Structure and Properties of the Fe-S Clusters inInorganic chemistry · 2026Article
- Rumen-derived Pichia membranifaciens modulates the rumen microbiome and metabolome and mitigates methane emissions in dairy cows.NPJ biofilms and microbiomes · 2026Article
- Metagenomic insight into the diversity and biogeochemical functions of microbial communities in the maar tropical Lake Atexcac.Microbiology (Reading, England) · 2026Article
- Probing the Role of Accessory FeS Clusters in Putative Sensory Group D [FeFe] Hydrogenase: Influence of the C-Terminal [4Fe-4S] Cluster on the Reactivity ofBiochemistry · 2026Article
- Atmospheric hydrogen consumption is regulated by glycerol-mediated catabolite repression in mycobacteria.mSystems · 2026Article
- Harnessing the early-life gut microbiome for sustainable ruminant production.Animal microbiome · 2026Review
- Comparative Genomics Reveals Unique Genetic Determinants of Biofilm Formation inInternational journal of molecular sciences · 2026Article
- Article
- Evolution and diversity of oxidoreductases involved in redox balance and energy conservation.Nature ecology & evolution · 2026Article
- Metagenomic analysis of fecal microbiomes reveals genetic potential for diverse hydrogen management strategies in marsupials.mSystems · 2026Article
- Cattle manure suppresses methane consumption and enhances denitrification-associated nitrous oxide production in farm dams.Microbiome · 2026Article
269 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent physiological and ecological studies have challenged the long-held belief that microbial metabolism of molecular hydrogen (H2) is a niche process. To gain a broader insight into the importance of microbial H2 metabolism, we comprehensively surveyed the genomic and metagenomic distribution of hydrogenases, the reversible enzymes that catalyse the oxidation and evolution of H2. The protein sequences of 3286 non-redundant putative hydrogenases were curated from publicly available databases. These metalloenzymes were classified into multiple groups based on (1) amino acid sequence phylogeny, (2) metal-binding motifs, (3) predicted genetic organisation and (4) reported biochemical characteristics. Four groups (22 subgroups) of [NiFe]-hydrogenase, three groups (6 subtypes) of [FeFe]-hydrogenases and a small group of [Fe]-hydrogenases were identified. We predict that this hydrogenase diversity supports H2-based respiration, fermentation and carbon fixation processes in both oxic and anoxic environments, in addition to various H2-sensing, electron-bifurcation and energy-conversion mechanisms. Hydrogenase-encoding genes were identified in 51 bacterial and archaeal phyla, suggesting strong pressure for both vertical and lateral acquisition. Furthermore, hydrogenase genes could be recovered from diverse terrestrial, aquatic and host-associated metagenomes in varying proportions, indicating a broad ecological distribution and utilisation. Oxygen content (pO2) appears to be a central factor driving the phylum- and ecosystem-level distribution of these genes. In addition to compounding evidence that H2 was the first electron donor for life, our analysis suggests that the great diversification of hydrogenases has enabled H2 metabolism to sustain the growth or survival of microorganisms in a wide range of ecosystems to the present day. This work also provides a comprehensive expanded system for classifying hydrogenases and identifies new prospects for investigating H2 metabolism.
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