Evidence map›Paper›PMID 26405822›Full record

ArticleJournal of biomedical optics2015

Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy.

Jing Liu, Nur P Damayanti, Il-Hoon Cho, Yesim Polar, Sunil Badve, Joseph M K Irudayaraj

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jing LiuPurdue University, Bindley Bioscience Center and Birck Nanotechnology Center, Agriculture and Biological Engineering, West Lafayette, Indiana 47907, United StatesdSouth Dakota School of Mines and Technology, Nanoscience and Nanoengineering, Rapid City, So.
Nur P DamayantiPurdue University, Bindley Bioscience Center and Birck Nanotechnology Center, Agriculture and Biological Engineering, West Lafayette, Indiana 47907, United States.
Il-Hoon ChoPurdue University, Bindley Bioscience Center and Birck Nanotechnology Center, Agriculture and Biological Engineering, West Lafayette, Indiana 47907, United StatesbEulji University, College of Health Science, Department of Biomedical Laboratory Science, Se.
Yesim PolarIndiana University School of Medicine, Department of Pathology and Laboratory Medicine, Indianapolis, Indiana 46202, United States.
Sunil BadveIndiana University School of Medicine, Department of Pathology and Laboratory Medicine, Indianapolis, Indiana 46202, United States.
Joseph M K IrudayarajPurdue University, Bindley Bioscience Center and Birck Nanotechnology Center, Agriculture and Biological Engineering, West Lafayette, Indiana 47907, United States.

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI Andrea L Kasinski · 1985 to 2026
$43.4M
NCI NIH HHS P30 CA023168
6 · The paper itself

Abstract

Fluorescence-based single molecule techniques to interrogate gene expression in tissues present a very low signal-to-noise ratio due to the strong autofluorescence and other background signals from tissue sections. This report presents a background-free method using second-harmonic generation (SHG) nanocrystals as probes to quantify the messenger RNA (mRNA) of human epidermal growth receptor 2 (Her2) at single molecule resolution in specific phenotypes at single-cell resolution directly in tissues. Coherent SHG emission from individual barium titanium oxide (BTO) nanoprobes was demonstrated, allowing for a stable signal beyond the autofluorescence window. Her2 surface marker and Her2 mRNA were specifically labeled with BTO probes, and Her2 mRNA was quantified at single copy sensitivity in Her2 expressing phenotypes directly in cancer tissues. Our approach provides the first proof of concept of a cross-platform strategy to probe tissues at single-cell resolution in situ.

Indexed as

Barium CompoundsBreast NeoplasmsFemaleGene Expression ProfilingHumansImage Processing, Computer-AssistedMetal NanoparticlesMicroscopyNeoplasmsRNA, MessengerSignal-To-Noise RatioSingle-Cell AnalysisTitaniumTranscriptomeBarium Compoundsbarium titanate(IV)RNA, MessengerTitanium

Identifiers

PMID26405822
PMCPMC4688913

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.