ArticleNeuroscience2015
Increased excitability and excitatory synaptic transmission during in vitro ischemia in the neonatal mouse hippocampus.
Article in Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Digitoids: a novel computational platform for mimicking oxygen-dependent firing of neuronsFrontiers in neuroinformatics · 2025Article
- Modelling hyperexcitability in human cerebral cortical organoids: Oxygen/glucose deprivation most effective stimulant.Heliyon · 2023Article
- Estradiol decreases the excitability of RA projection neurons in adult male zebra finches.Frontiers in cellular neuroscience · 2023Article
- Maternal Hypoxia Increases the Excitability of Neurons in the Entorhinal Cortex and Dorsal Hippocampus of Rat Offspring.Frontiers in neuroscience · 2022Article
- Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.Neural regeneration research · 2022Article
- Article
- On the origin of ultraslow spontaneous NaJournal of neurophysiology · 2021Article
- Neocortical injury-induced status epilepticus.Epilepsia · 2020Article
- Increased Excitability and Heightened Magnitude of Long-Term Potentiation at Hippocampal CA3-CA1 Synapses in a Mouse Model of Neonatal Hyperoxia Exposure.Frontiers in synaptic neuroscience · 2020Article
- Tissue Injury and Astrocytic Reaction, But Not Cognitive Deficits, Are Dependent on Hypoxia Duration in Very Immature Rats Undergoing Neonatal Hypoxia-Ischemia.Neurochemical research · 2019Article
- Immediate and delayed decrease of long term potentiation and memory deficits after neonatal intermittent hypoxia.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2019Article
- The Role of Kainate Receptors in the Pathophysiology of Hypoxia-Induced Seizures in the Neonatal Mouse.Scientific reports · 2018Article
- Molecular chaperones and hypoxic-ischemic encephalopathy.Neural regeneration research · 2017Review
- Optimized Model of Cerebral IschemiaFrontiers in neuroscience · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThe present study tested the hypothesis that exposure to in vitro hypoxia-ischemia alters membrane properties and excitability as well as excitatory synaptic transmission of CA1 pyramidal neurons in the neonatal mouse.
methodsExperiments were conducted in hippocampal slices in P7-P9 C57Bl/6 mice using whole-cell patch clamp in current- and voltage-clamp mode. Passive membrane potential (Vm), input resistance (Rin) and active (action potential (AP) threshold and amplitude) membrane properties of CA1 pyramidal neurons were assessed at baseline, during 10 min in vitro ischemia (oxygen-glucose deprivation (OGD)) and during reoxygenation. Spontaneous and miniature excitatory post-synaptic currents (s and mEPSCs) were studied under similar conditions.
resultsOGD caused significant depolarization of CA1 pyramidal neurons as well as decrease in AP threshold and increase in AP amplitude. These changes were blocked by the application of tetrodotoxin (TTX), indicating Na(+) channels' involvement. Following 10 min of reoxygenation, significant membrane hyperpolarization was noted and it was associated with a decrease in Rin. AP threshold and amplitude returned to baseline during that stage. sEPSC and mEPSC frequency increased during both OGD and reoxygenation but their amplitude remained unchanged. Additionally, we found that OGD decreases Ih (hyperpolarization activated current) in CA1 neurons from neonatal mice and this effect persists during reoxygenation. SIGNIFICANCE: These results indicate that in vitro ischemia leads to changes in membrane excitability mediated by sodium and potassium channels. Further, it results in enhanced neurotransmitter release from presynaptic terminals. These changes are likely to represent one of the mechanisms of hypoxia/ischemia-mediated seizures in the neonatal period.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.