ArticleBMC veterinary research2015
In vitro effects of 3% hypertonic saline and 20% mannitol on canine whole blood coagulation and platelet function.
Article in BMC veterinary research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- Comparison of the effects of 7.2% hypertonic saline and 20% mannitol on whole blood coagulation and platelet function in dogs with suspected intracranial hypertension - a pilot study.BMC veterinary research · 2017Trial
- Impact of general anesthesia on rotational thromboelastometry (ROTEM) parameters and standard plasmatic coagulation tests in healthy Beagle dogs.Veterinary and animal science · 2021Article
- Effects of Blood Components and Whole Blood in a Model of Severe Trauma-Induced Coagulopathy.The Journal of surgical research · 2021Article
- Intravenous Fluid Administration and the Coagulation System.Frontiers in veterinary science · 2021Review
- Effect of 7.5% hypertonic saline solution on whole blood coagulation in healthy dogs using thromboelastography.Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001) · 2020Article
- Examining the Effect of Hypertonic Saline Administered for Reduction of Intracranial Hypertension on Coagulation.Journal of the American College of Surgeons · 2020Article
- Mannitol and the Combination of Mannitol and Gelatin Impair Whole Blood Coagulation and the Platelet FunctionTurkish journal of anaesthesiology and reanimation · 2019Article
- Respective Contributions of Head and Extra-Head Injury Severities to the Occurrence of Early Coagulopathy Following Traumatic Brain Injury.Neurotrauma reportsArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHyperosmolar therapy, using either mannitol or hypertonic saline (HTS), is considered the treatment of choice for intracranial hypertension. However, hyperosmolar agents may impair coagulation and platelet function, limiting their use in patients at risk for hemorrhage. Despite this, studies evaluating the effects of mannitol compared to other hyperosmolar agents in dogs are largely lacking. The aim of this study was to compare the in vitro effects on global hemostasis and platelet function of 20% mannitol and 3% HTS on canine blood.
methodsCitrated whole blood from 15 healthy dogs was diluted with 0.9% saline, 20% mannitol and 3% HTS in ratios of 1:16 and 1:8. Rotational thromboelastometry (ROTEM) was used to assess clotting time (CT), clot formation time (CFT) and maximal clot firmness (MCF) following extrinsic activation (Ex-tem) and after platelet inhibition (Fib-tem). A platelet function analyzer (PFA-100) was used to assess closure time (Ct(PFA)).
resultsNo significant differences were observed between untreated whole blood and samples diluted with saline. Samples diluted with both mannitol and HTS were hypocoagulable compared to untreated whole blood samples. At a dilution of 1:16, no significant differences were found between any measured parameter in samples diluted with saline compared to mannitol or HTS. At a 1:8 dilution, Ct(PFA) was prolonged in samples diluted with mannitol and HTS compared to saline, and Ct(PFA) was prolonged more with mannitol than HTS. Ex-tem CT was increased at a 1:8 dilution with mannitol compared to HTS. Ex-tem CFT was prolonged at a 1:8 dilution with both agents compared to saline, and was prolonged more with mannitol than HTS. Ex-tem MCF was reduced at a 1:8 dilution with both agents compared to saline. DISCUSSION AND
conclusionsData in this study indicate that both mannitol and HTS affect canine platelet function and whole blood coagulation in vitro in a dose-dependent fashion. The most pronounced effects were observed after high dilutions with mannitol, which impaired platelet aggregation, clot formation time, clot strength, and fibrin formation significantly more than HTS. Further in vivo studies are necessary before recommendations can be made.
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