Evidence map›Paper›PMID 26402844›Full record

ArticleEndocrinology2015

Diversity of Reporter Expression Patterns in Transgenic Mouse Lines Targeting Corticotropin-Releasing Hormone-Expressing Neurons.

Yuncai Chen, Jenny Molet, Benjamin G Gunn, Kerry Ressler, Tallie Z Baram

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 102 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yuncai ChenDepartments of Pediatrics (Y.C., B.G.G., T.Z.B.) and Anatomy/Neurobiology (Y.C., J.M., B.G.G., T.Z.B.), University of California, Irvine, Irvine, California 92697-4475; and Department of Psychiatry and Behavioral Sciences (K.R.), Emory University, Atlanta, Georgia 30322-4250.
Jenny MoletDepartments of Pediatrics (Y.C., B.G.G., T.Z.B.) and Anatomy/Neurobiology (Y.C., J.M., B.G.G., T.Z.B.), University of California, Irvine, Irvine, California 92697-4475; and Department of Psychiatry and Behavioral Sciences (K.R.), Emory University, Atlanta, Georgia 30322-4250.
Benjamin G GunnDepartments of Pediatrics (Y.C., B.G.G., T.Z.B.) and Anatomy/Neurobiology (Y.C., J.M., B.G.G., T.Z.B.), University of California, Irvine, Irvine, California 92697-4475; and Department of Psychiatry and Behavioral Sciences (K.R.), Emory University, Atlanta, Georgia 30322-4250.
Kerry ResslerDepartments of Pediatrics (Y.C., B.G.G., T.Z.B.) and Anatomy/Neurobiology (Y.C., J.M., B.G.G., T.Z.B.), University of California, Irvine, Irvine, California 92697-4475; and Department of Psychiatry and Behavioral Sciences (K.R.), Emory University, Atlanta, Georgia 30322-4250.
Tallie Z BaramDepartments of Pediatrics (Y.C., B.G.G., T.Z.B.) and Anatomy/Neurobiology (Y.C., J.M., B.G.G., T.Z.B.), University of California, Irvine, Irvine, California 92697-4475; and Department of Psychiatry and Behavioral Sciences (K.R.), Emory University, Atlanta, Georgia 30322-4250.
University of California, Irvine · USEmory University · US

Funding

Structural & Network-Function Correlates of Fragmented Early-Life Across SpeciesP50MH096889 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARAM, TALLIE Z. · 2013 to 2023
$25.4M
Effects of Early-Life Experience: Role of CRHR01MH073136 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARAM, TALLIE Z. · 2005 to 2020
$4.6M
CRH-MRNA REGULATION--ROLE IN NEONATAL SEIZURE POTENTIALR01NS028912 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARAM, TALLIE Z. · 1992 to 2014
$4.2M
NIMH NIH HHS MH73136NIMH NIH HHS P50 MH096889NIMH NIH HHS R01 MH073136NINDS NIH HHS NS28912NINDS NIH HHS R01 NS028912
6 · The paper itself

Abstract

Transgenic mice, including lines targeting corticotropin-releasing factor (CRF or CRH), have been extensively employed to study stress neurobiology. These powerful tools are poised to revolutionize our understanding of the localization and connectivity of CRH-expressing neurons, and the crucial roles of CRH in normal and pathological conditions. Accurate interpretation of studies using cell type-specific transgenic mice vitally depends on congruence between expression of the endogenous peptide and reporter. If reporter expression does not faithfully reproduce native gene expression, then effects of manipulating unintentionally targeted cells may be misattributed. Here, we studied CRH and reporter expression patterns in 3 adult transgenic mice: Crh-IRES-Cre;Ai14 (tdTomato mouse), Crfp3.0CreGFP, and Crh-GFP BAC. We employed the CRH antiserum generated by Vale after validating its specificity using CRH-null mice. We focused the analyses on stress-salient regions, including hypothalamus, amygdala, bed nucleus of the stria terminalis, and hippocampus. Expression patterns of endogenous CRH were consistent among wild-type and transgenic mice. In tdTomato mice, most CRH-expressing neurons coexpressed the reporter, yet the reporter identified a few non-CRH-expressing pyramidal-like cells in hippocampal CA1 and CA3. In Crfp3.0CreGFP mice, coexpression of CRH and the reporter was found in central amygdala and, less commonly, in other evaluated regions. In Crh-GFP BAC mice, the large majority of neurons expressed either CRH or reporter, with little overlap. These data highlight significant diversity in concordant expression of reporter and endogenous CRH among 3 available transgenic mice. These findings should be instrumental in interpreting important scientific findings emerging from the use of these potent neurobiological tools.

Indexed as

AmygdalaAnimalsBrainCorticotropin-Releasing HormoneGenes, ReporterGreen Fluorescent ProteinsHippocampusHypothalamusInternal Ribosome Entry SitesMaleMiceMice, TransgenicNeuronsParaventricular Hypothalamic NucleusRNA, MessengerSeptal NucleiCorticotropin-Releasing HormoneGreen Fluorescent ProteinsInternal Ribosome Entry SitesRNA, Messenger

Identifiers

PMID26402844
PMCPMC4655217
OpenAlexW2177640580

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.