ArticleMicrobial biotechnology2016
EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785.
Article in Microbial biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Women's health is a team effort: probiogenomics supports the development of a multi-species vaginal probiotic.Cellular and molecular life sciences : CMLS · 2026Article
- New progress in the identifying regulatory factors of exopolysaccharide synthesis in lactic acid bacteria.World journal of microbiology & biotechnology · 2023Review
- Whole genome sequence ofFrontiers in microbiology · 2023Article
- Shotgun metagenomic sequencing revealed the prebiotic potential of a grain-based diet in mice.Scientific reports · 2022Article
- Diverse functions for acyltransferase-3 proteins in the modification of bacterial cell surfaces.Microbiology (Reading, England) · 2022Article
- Differentiation ofFoods (Basel, Switzerland) · 2021Article
- Assessment ofJournal of microbiology and biotechnology · 2021Article
- Article
- The second messenger c-di-AMP mediates bacterial exopolysaccharide biosynthesis: a review.Molecular biology reports · 2020Review
- Identification of Genes Required for Glucan Exopolysaccharide Production in Lactobacillus johnsonii Suggests a Novel Biosynthesis Mechanism.Applied and environmental microbiology · 2020Article
- Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.Current microbiology · 2020Article
- Comparative Genomics Analysis ofGenes · 2020Article
- A Diverse Repertoire of Exopolysaccharide Biosynthesis Gene Clusters inMicroorganisms · 2019Article
- Contribution of YthA, a PspC Family Transcriptional Regulator of Lactococcus lactis F44 Acid Tolerance and Nisin Yield: a Transcriptomic Approach.Applied and environmental microbiology · 2018Article
- Bacteria Facilitate Enteric Virus Co-infection of Mammalian Cells and Promote Genetic Recombination.Cell host & microbe · 2018Article
- Interactions of Surface Exopolysaccharides FromFrontiers in microbiology · 2018Review
- EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785.Microbial biotechnology · 2016Article
- Intestinal microbial communities andGut microbesArticle
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4 authors.
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Abstract
Lactobacillus johnsonii FI9785 has an eps gene cluster which is required for the biosynthesis of homopolymeric exopolysaccharides (EPS)-1 and heteropolymeric EPS-2 as a capsular layer. The first gene of the cluster, epsA, is the putative transcriptional regulator. In this study we showed the crucial role of epsA in EPS biosynthesis by demonstrating that deletion of epsA resulted in complete loss of both EPS-1 and EPS-2 on the cell surface. Plasmid complementation of the epsA gene fully restored EPS production, as confirmed by transmission electron microscopy and nuclear magnetic resonance (NMR) analysis. Furthermore, this complementation resulted in a twofold increase in the expression levels of this gene, which almost doubled amounts of EPS production in comparison with the wild-type strain. Analysis of EPS by NMR showed an increased ratio of the heteropolysaccharide to homopolysaccharide in the complemented strain and allowed identification of the acetylated residue in EPS-2 as the (1,4)-linked βGlcp unit, with the acetyl group located at O-6. These findings indicate that epsA is a positive regulator of EPS production and that EPS production can be manipulated by altering its expression.
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