ArticleHuman molecular genetics2015
The cumulative effect of assisted reproduction procedures on placental development and epigenetic perturbations in a mouse model.
Article in Human molecular genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
91 citing papers in PubMed, 2 syntheses or guidelines pooled it, 158 citations in OpenAlex.
- A systematic review of genome-wide analyses of methylation changes associated with assisted reproductive technologies in various tissues.Fertility and sterility · 2024Pooled it
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Epigenetic, environmental, and sex-specific programming of long-term health in Assisted Reproductive Technologies-conceived offspring†.Biology of reproduction · 2026Review
- The Fallopian tube: Biosensor and dynamic regulator of preimplantation development.PNAS nexus · 2026Review
- Ultra-low oxygen tension duringbioRxiv : the preprint server for biology · 2026Article
- Inhibition of p65 nuclear translocation in decidual stromal cells underlies COS-induced supraphysiologic estrogen impairment of uNK cell function which drives placental abnormalities.Journal of translational medicine · 2026Article
- Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†.Biology of reproduction · 2026Article
- Modest increase in the de novo single-nucleotide mutation rate in house mice born by assisted reproduction.Genome research · 2026Article
- Reappraisal of adverse obstetric outcomes independently associated with contemporary fertility treatment practices: a US prospective cohort study.American journal of obstetrics and gynecology · 2026Article
- Differential Effects of Assisted Reproduction Technology on Placental Epigenetics and Angiogenesis: Insights from Fresh, Frozen, and Egg Donation Pregnancies.Life (Basel, Switzerland) · 2025Article
- The impact of melatonin-enriched media on epigenetic and perinatal changes induced by embryo culture in a mouse model.Journal of assisted reproduction and genetics · 2025Article
- Review
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- Unmasking the Epigenome: Insights into Testicular Cell Dynamics and Reproductive Function.International journal of molecular sciences · 2025Review
- Increased rate ofbioRxiv : the preprint server for biology · 2025Article
- In vitro fertilization induces reproductive changes in male mouse offspring and has multigenerational effects.JCI insight · 2025Article
- Review
- Loci associated with spontaneous abortion in primiparous Holstein cattle.Frontiers in veterinary science · 2025Article
- Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.Epigenomics · 2025Article
- Genome-wide DNA methylation and gene expression in human placentas derived from assisted reproductive technology.Communications medicine · 2024Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Assisted reproductive technologies (ART) are associated with several complications including low birth weight, abnormal placentation and increased risk for rare imprinting disorders. Indeed, experimental studies demonstrate ART procedures independent of existing infertility induce epigenetic perturbations in the embryo and extraembryonic tissues. To test the hypothesis that these epigenetic perturbations persist and result in adverse outcomes at term, we assessed placental morphology and methylation profiles in E18.5 mouse concepti generated by in vitro fertilization (IVF) in two different genetic backgrounds. We also examined embryo transfer (ET) and superovulation procedures to ascertain if they contribute to developmental and epigenetic effects. Increased placental weight and reduced fetal-to-placental weight ratio were observed in all ART groups when compared with naturally conceived controls, demonstrating that non-surgical embryo transfer alone can impact placental development. Furthermore, superovulation further induced overgrowth of the placental junctional zone. Embryo transfer and superovulation defects were limited to these morphological changes, as we did not observe any differences in epigenetic profiles. IVF placentae, however, displayed hypomethylation of imprinting control regions of select imprinted genes and a global reduction in DNA methylation levels. Although we did not detect significant differences in DNA methylation in fetal brain or liver samples, rare IVF concepti displayed very low methylation and abnormal gene expression from the normally repressed allele. Our findings suggest that individual ART procedures cumulatively increase placental morphological abnormalities and epigenetic perturbations, potentially causing adverse neonatal and long-term health outcomes in offspring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.