ArticleProceedings of the National Academy of Sciences of the United States of America2015
Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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96 citing papers in PubMed.
- Loss of Nod25 function does not affect nitrogen-fixing symbiosis in Medicago truncatula.Plant molecular biology · 2026Article
- ESN3, a nodule-specific small peptide essential for symbiotic nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
- Engineering Symbiotic Nitrogen Fixation for Agriculture: Predominant Role of Host Plants and Fine-Tuning Regulation.Plants (Basel, Switzerland) · 2026Article
- BacA(SbmA) importer of legume symbiotic NCR peptides: Protein architecture, function, and evolutionary implications.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nodule-Microbiome Dynamics: Deciphering the Complexities of Nodule Symbiosis and the Root Microbiome.International journal of molecular sciences · 2026Review
- Article
- Mutations in legume genes that influence symbiosis create a complex selective landscape for rhizobial symbionts.The ISME journal · 2026Article
- Impacts of gene duplication in the evolution of symbiotic root nodule symbiosis in legumes.Frontiers in plant science · 2026Article
- A plant-derived antimicrobial peptide with multiple mechanisms of action exhibiting antibacterial and antibiofilm activities comparable to or superior to polymyxin B.Current research in microbial sciences · 2026Article
- Inhibition of rhizobial cheaters by the host Medicago truncatula involves repression of symbiotic functions and induction of defense.The New phytologist · 2025Article
- Uclacyanin MtUC1 Is Involved in the Regulation of Nodule Senescence in Medicago truncatula.Molecular plant pathology · 2025Article
- Diverse triggers, common outcome: Senescence in Fix⁻ Medicago truncatula nodules.Plant physiology · 2025Article
- Mobile gene clusters and coexpressed plant-rhizobium pathways drive partner quality variation in symbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A minimized symbiotic gene set from the 1.68 Mb pSymB chromid of Sinorhizobium meliloti reveals auxiliary symbiotic loci.BMC biology · 2025Article
- Article
- Protein import into bacterial endosymbionts and evolving organelles.The FEBS journal · 2025Review
- Enacting partner specificity in legume-rhizobia symbioses.aBIOTECH · 2025Review
- The Small Key to the Treasure Chest: Endogenous Plant Peptides Involved in Symbiotic Interactions.Plants (Basel, Switzerland) · 2025Review
- A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules.Scientific reports · 2024Article
- Two members of a Nodule-specific Cysteine-Rich (NCR) peptide gene cluster are required for differentiation of rhizobia in Medicago truncatula nodules.The Plant journal : for cell and molecular biology · 2024Article
36 more citing papers are in PubMed but not listed here.
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13 authors.
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Abstract
Host compatible rhizobia induce the formation of legume root nodules, symbiotic organs within which intracellular bacteria are present in plant-derived membrane compartments termed symbiosomes. In Medicago truncatula nodules, the Sinorhizobium microsymbionts undergo an irreversible differentiation process leading to the development of elongated polyploid noncultivable nitrogen fixing bacteroids that convert atmospheric dinitrogen into ammonia. This terminal differentiation is directed by the host plant and involves hundreds of nodule specific cysteine-rich peptides (NCRs). Except for certain in vitro activities of cationic peptides, the functional roles of individual NCR peptides in planta are not known. In this study, we demonstrate that the inability of M. truncatula dnf7 mutants to fix nitrogen is due to inactivation of a single NCR peptide, NCR169. In the absence of NCR169, bacterial differentiation was impaired and was associated with early senescence of the symbiotic cells. Introduction of the NCR169 gene into the dnf7-2/NCR169 deletion mutant restored symbiotic nitrogen fixation. Replacement of any of the cysteine residues in the NCR169 peptide with serine rendered it incapable of complementation, demonstrating an absolute requirement for all cysteines in planta. NCR169 was induced in the cell layers in which bacteroid elongation was most pronounced, and high expression persisted throughout the nitrogen-fixing nodule zone. Our results provide evidence for an essential role of NCR169 in the differentiation and persistence of nitrogen fixing bacteroids in M. truncatula.
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