ArticleProceedings of the National Academy of Sciences of the United States of America2015
Dynamic label-free imaging of lipid nanodomains.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 120 citations in OpenAlex.
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Deciphering Evolution, Function, and Observation of Crystallization-Driven Self-Assembly.Chemical reviews · 2025Review
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Potency of agarose gel-supported lipid bilayers for electrophysiologic analysis of channel pores formed by Bacillus thuringiensis insecticidal proteins.The FEBS journal · 2025Article
- Real-time label-free imaging of living crystallization-driven self-assembly.Nature communications · 2025Article
- One-dimensional photonic crystal enhancing spin-to-orbital angular momentum conversion for single-particle tracking.Light, science & applications · 2024Article
- Measuring the Transmembrane Registration of Lipid Domains in Droplet Interface Bilayers through Tensiometry.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Nature communications · 2024Article
- Multiscale Modeling of Macromolecular Interactions between Tau-Amylin Oligomers and Asymmetric Lipid Nanodomains That Link Alzheimer's and Diabetic Diseases.Molecules (Basel, Switzerland) · 2024Article
- Heterogeneous biological membranes regulate protein partitioning via fluctuating diffusivity.PNAS nexus · 2023Article
- Eukaryotic Cell Membranes: Structure, Composition, Research Methods and Computational Modelling.International journal of molecular sciences · 2023Review
- Multiplexed Protein Detection and Parallel Binding Kinetics Analysis with Label-Free Digital Single-Molecule Counting.Analytical chemistry · 2023Article
- Exploring Membrane Binding Targets of Disordered Human Tau Aggregates on Lipid Rafts Using Multiscale Molecular Dynamics Simulations.Membranes · 2022Article
- Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope.Membranes · 2022Article
- Challenges and opportunities in achieving the full potential of droplet interface bilayers.Nature chemistry · 2022Review
- Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature.Biophysical reviews · 2022Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Dynamic "Molecular Portraits" of Biomembranes Drawn by Their Lateral Nanoscale Inhomogeneities.International journal of molecular sciences · 2021Review
- Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins.iScience · 2021Article
- Direct label-free imaging of nanodomains in biomimetic and biological membranes by cryogenic electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid rafts are submicron proteolipid domains thought to be responsible for membrane trafficking and signaling. Their small size and transient nature put an understanding of their dynamics beyond the reach of existing techniques, leading to much contention as to their exact role. Here, we exploit the differences in light scattering from lipid bilayer phases to achieve dynamic imaging of nanoscopic lipid domains without any labels. Using phase-separated droplet interface bilayers we resolve the diffusion of domains as small as 50 nm in radius and observe nanodomain formation, destruction, and dynamic coalescence with a domain lifetime of 220±60 ms. Domain dynamics on this timescale suggests an important role in modulating membrane protein function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.