ArticleJournal of clinical microbiology2015
Core Genome Multilocus Sequence Typing Scheme for High- Resolution Typing of Enterococcus faecium.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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Who cites it
181 citing papers in PubMed.
- Emergence of a Novel, Phenotypically Difficult-to-Detect Vancomycin-Resistant Enterococcus faecium Clone (ST117/CT7799).MicrobiologyOpen · 2026Article
- Performance of disk diffusion and MIC gradient tests in tigecycline susceptibility testing of enterococci: a Nordic multicentre study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Comparative genomic characterization and antimicrobial resistance of bacteremia-causingMicrobiology spectrum · 2026Article
- Recombination and accessory genome turnover shaped the rise and decline of vanA Enterococcus faecium ST80 in Denmark.Genome medicine · 2026Article
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- Comparing whole-genome sequencing to pulsed-field gel electrophoresis for vancomycin-resistantMicrobiology spectrum · 2026Article
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- Evaluation of Oxford Nanopore Technologies workflows for genomic epidemiology of outbreak-associated bacterial isolates in the clinical setting.Microbial genomics · 2026Article
- Regional VRE surveillance using routine centralised, multicentre whole genome sequencing.PloS one · 2026Article
- One-year multicenter surveillance of Fosfomycin resistance Enterobacterales: the rise of FosA3-producingCurrent research in microbial sciences · 2026Article
- Molecular epidemiology of vancomycin resistant enterococci collected from 9 hospitals in Beijing, China: a multicenter study.Frontiers in cellular and infection microbiology · 2026Article
- Rapid Detection of VREfm Clusters: FTIR Spectroscopy as a Practical Alternative to Whole-Genome Sequencing.Open forum infectious diseases · 2025Article
- The potential of genome-oriented blood culture surveillance of vancomycin-resistant enterococcus faecium (VRE) to mirror local VRE epidemiology: a retrospective analysis and systematic comparison of VRE blood culture and VRE first patient isolates.BMC infectious diseases · 2025Article
- Comparative analysis of vancomycin-resistant enterococci in colonization and infection-a longitudinal study.Microbiology spectrum · 2025Article
- Global epidemiological insights on the antimicrobial resistance ofMicrobial genomics · 2025Article
- Emergence of vancomycin-resistantEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Development and validation of a core genome multilocus sequence typing scheme forJournal of clinical microbiology · 2025Article
- Virulence signature of the endemic vancomycin-resistantMicrobiology spectrum · 2025Article
121 more citing papers are in PubMed but not listed here.
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14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug-resistant nosocomial pathogen. Since the start of the 21st century, multilocus sequence typing (MLST) has been used to study the molecular epidemiology of E. faecium. However, due to the use of a small number of genes, the resolution of MLST is limited. Whole-genome sequencing (WGS) now allows for high-resolution tracing of outbreaks, but current WGS-based approaches lack standardization, rendering them less suitable for interlaboratory prospective surveillance. To overcome this limitation, we developed a core genome MLST (cgMLST) scheme for E. faecium. cgMLST transfers genome-wide single nucleotide polymorphism(SNP) diversity into a standardized and portable allele numbering system that is far less computationally intensive than SNP-based analysis of WGS data. The E. faecium cgMLST scheme was built using 40 genome sequences that represented the diversity of the species. The scheme consists of 1,423 cgMLST target genes. To test the performance of the scheme, we performed WGS analysis of 103 outbreak isolates from five different hospitals in the Netherlands, Denmark, and Germany. The cgMLST scheme performed well in distinguishing between epidemiologically related and unrelated isolates, even between those that had the same sequence type (ST), which denotes the higher discriminatory power of this cgMLST scheme over that of conventional MLST. We also show that in terms of resolution, the performance of the E. faecium cgMLST scheme is equivalent to that of an SNP-based approach. In conclusion, the cgMLST scheme developed in this study facilitates rapid, standardized, and high-resolution tracing of E. faecium outbreaks.
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