ArticleExtremophiles : life under extreme conditions2015
A reporter gene system for the precise measurement of promoter activity in Thermus thermophilus HB27.
Article in Extremophiles : life under extreme conditions, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- A robust enzymatic reporter system for the extremely thermophilic anaerobic bacteriumFrontiers in microbiology · 2026Article
- Sugar-inducible promoters for manipulation of core metabolic pathways in the thermophilic acetogenApplied and environmental microbiology · 2025Article
- Engineering artificial 5' regulatory sequences for thermostable protein expression in the extremophileSynthetic biology (Oxford, England) · 2025Article
- Article
- Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant.Scientific reports · 2021Article
- Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter.Microbial cell factories · 2020Article
- Article
- Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus.Molecular genetics and genomics : MGG · 2018Article
- Development of a new host-vector system for colour selection of cloned DNA inserts using a newly designed β-galactosidase gene containing multiple cloning sites in Thermus thermophilus HB27.Extremophiles : life under extreme conditions · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a reporter gene system that enables precise analysis of promoter activity in Thermus thermophilus HB27. The reporter vector employs a promoterless β-galactosidase gene of Thermus spp. strain T2. However, T. thermophilus HB27 strain has three genes (TTP0042, TTP0220 and TTP0222) whose products have β-galactosidase activity, which would interfere with correct measurements of promoter activities. Thus, to eliminate this background activity, we disrupted all three of these genes to generate a host strain for measuring promoter expression as β-galactosidase activity. In addition, T. thermophilus strains also produce carotenoids called thermoxanthins that are yellow pigments. To avoid the influence of these carotenoids on the β-galactosidase assay, we also disrupted the phytoene synthase gene (crtB). The reporter gene system developed here is a powerful tool for studying transcriptional activity and the mechanisms that regulate gene expression in T. thermophilus HB27. We also showed that the crtB gene cassette could be used in repeated gene-disruption experiments to screen transformants by colony colour, thus eliminating the need for antibiotic resistance markers.
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