ArticleScientific reports2015
Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
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Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The ethics of genome editing in non-human animals: a systematic review of reasons reported in the academic literature.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Pooled it
- Effects of Partial Myostatin Loss of Function on Carcass Yield and Meat Quality in Pigs Differing in IGF2 Genotype.Animals : an open access journal from MDPI · 2026Article
- Targeting Myostatin for Sustainable Meat Production: Insights From Multiple Species.Animal genetics · 2026Review
- Early Growth and Serum Metabolic Profiling of One-Month-Old MSTN-Knockout Xinjiang Brown Cattle via CRISPR/Cas12Mix.Animals : an open access journal from MDPI · 2026Article
- A 90-day safety study of meat from MSTN gene-edited Mongolian cattle in mice.Scientific reports · 2026Article
- Harnessing myostatin pleiotropy for multitrait improvement via precision gene editing.Frontiers in genome editing · 2026Review
- Pig Genome Editing for Agriculture: Achievements and Challenges.International journal of molecular sciences · 2025Review
- A new strategy to reduce human influenza infection: creating pigs with partial resistance to influenza A virus infection by targetingJournal of biomedical research · 2025Article
- A bibliometric mapping of advancements and trends in genome editing in pigs.Tropical animal health and production · 2025Review
- VDGE: a data repository of variation database for gene-edited animals across multiple species.Nucleic acids research · 2025Article
- Diet supplemented with fermented onion improves growth performance, health condition, meat quality, and modifies rumen metabolite profiles in Liangshan black sheep.Frontiers in veterinary science · 2025Article
- Transcriptome and Metabolome Insights into Key Genes Regulating Fat Deposition and Meat Quality in Pig Breeds.Animals : an open access journal from MDPI · 2024Article
- AeLife · 2024Article
- Myostatin gene role in regulating traits of poultry species for potential industrial applications.Journal of animal science and biotechnology · 2024Review
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- Roles of Myokines and Muscle-Derived Extracellular Vesicles in Musculoskeletal Deterioration under Disuse Conditions.Metabolites · 2024Review
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Effects of Different Generations and Sex on Physiological, Biochemical, and Growth Parameters of Crossbred Beef Cattle byAnimals : an open access journal from MDPI · 2023Article
- Effects of Myostatin b Knockout on Offspring Body Length and Skeleton in Yellow Catfish (Biology · 2023Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs are an important source of meat production, as well as serve as a preferred animal model for the studies of human disease. To study the impacts of MSTN mutations on skeletal muscle growth in pigs, we generated MSTN-mutant Meishan pigs with no marker gene via zinc finger nucleases (ZFN) technology. The MSTN-mutant pigs developed and grew normally, had increased muscle mass with decreased fat accumulation compared with wild type pigs, and homozygote MSTN mutant (MSTN(-/-)) pigs had apparent DM phenotype, and individual muscle mass increased by 100% over their wild-type controls (MSTN(+/+)) at eight months of age as a result of myofiber hyperplasia. Interestingly, 20% MSTN-mutant pigs had one extra thoracic vertebra. The MSTN-mutant pigs will not only offer a way of fast genetic improvement of lean meat for local fat-type indigenous pig breeds, but also serve as an important large animal model for biomedical studies of musculoskeletal formation, development and diseases.
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