ArticleScientific reports2015
Enhanced nematicidal potential of the chitinase pachi from Pseudomonas aeruginosa in association with Cry21Aa.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 63 citations in OpenAlex.
- An artificial selection procedure enriches for known and suspected chitin degraders from the prokaryotic rare biosphere of multiple marine biotopes.BMC microbiology · 2025Article
- Interspecies systems biology links bacterial metabolic pathways to nematode gene expression, chemotaxis behavior, and survival.Genome research · 2025Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Rational Design of a Potential New Nematicide Targeting Chitin Deacetylase.Journal of agricultural and food chemistry · 2024Article
- Article
- Larvicidal, growth inhibitory and biochemical effects of soil bacterium, Pseudomonas sp. EN4 against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae).BMC microbiology · 2023Article
- Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy againstPeerJ · 2023Article
- Characterization of Nematicidal Activity and Nematode-Toxic Metabolites of a SoilbornePathogens (Basel, Switzerland) · 2022Article
- Insecticidal and growth inhibitory activity of gut microbes isolated from adults of Spodoptera litura (Fab.).BMC microbiology · 2022Article
- Serratia sp., an endophyte of Mimosa pudica nodules with nematicidal, antifungal activity and growth-promoting characteristics.Archives of microbiology · 2021Article
- Thermophilic Chitinases: Structural, Functional and Engineering Attributes for Industrial Applications.Applied biochemistry and biotechnology · 2021Review
- Combating Parasitic Nematode Infections, Newly Discovered Antinematode Compounds from Marine Epiphytic Bacteria.Microorganisms · 2020Review
- Chitinase production by Trichoderma koningiopsis UFSMQ40 using solid state fermentation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- TOXiTAXi: a web resource for toxicity of Bacillus thuringiensis protein compositions towards species of various taxonomic groups.Scientific reports · 2020Article
- Article
- The Nif3-Family Protein YqfO03 fromInternational journal of molecular sciences · 2018Article
- Rhizobacteria with nematicide aptitude: enzymes and compounds associated.World journal of microbiology & biotechnology · 2016Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nematodes are known to be harmful to various crops, vegetables, plants and insects. The present study reports that, chitin upregulates the activity of chitinase (20%) and nematicidal potential (15%) of Pseudomonas aeruginosa. The chitinase gene (pachi) from P. aeruginosa was cloned, and its nematicidal activity of pachi protein against Caenorhabditis elegans was studied. The mortality rate induced by pachi increased by 6.3-fold when in association with Cry21Aa from Bacillus thuringiensis. Pachi efficiently killed C. elegans in its native state (LC50 = 387.3 ± 31.7 μg/ml), as well as in association with Cry21Aa (LC50 = 30.9 ± 4.1 μg/ml), by degrading the cuticle, egg shell and intestine in a relatively short time period of 24 h. To explore the nematidal potential of chitinase, six fusion proteins were constructed using gene engineering techniques. The CHACry showed higher activity against C. elegans than others owing to its high solubility. Notably, the CHACry showed a synergistic factor of 4.1 versus 3.5 a mixture [1:1] of pachi and Cry21Aa. The present study has identified eco-friendly biological routes (e.g., mixed proteins, fusion proteins) with potent nematicidal activity, which not only can help to prevent major crop losses but also strengthen the agro-economy and increase gross crop yield.
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