ArticleAnnals of biomedical engineering2016
Analyzing Remodeling of Cardiac Tissue: A Comprehensive Approach Based on Confocal Microscopy and 3D Reconstructions.
Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- A deep learning-enabled toolkit for the 3D segmentation of ventricular cardiomyocytes.The Journal of physiology · 2026Article
- Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.Microcirculation (New York, N.Y. : 1994) · 2025Article
- Unveiling the native architecture of adult cardiac tissue using the 3D-NaissI method.Cellular and molecular life sciences : CMLS · 2025Article
- Pericytes in mouse heart.Frontiers in physiology · 2025Article
- Adeno-associated virus-mediated gene delivery ofAmerican journal of physiology. Heart and circulatory physiology · 2024Article
- Effects of electro-mechanical uncouplers, hormonal stimulation and pacing rate on the stability and function of cultured rabbit myocardial slices.Frontiers in bioengineering and biotechnology · 2024Article
- Contractility measurements for cardiotoxicity screening with ventricular myocardial slices of pigs.Cardiovascular research · 2023Article
- Cardiomyocyte orientation recovery at micrometer scale reveals long-axis fiber continuum in heart walls.The EMBO journal · 2023Article
- Ryanodine Receptor Staining Identifies Viable Cardiomyocytes in Human and Rabbit Cardiac Tissue Slices.International journal of molecular sciences · 2023Article
- New Insights into the Reparative Angiogenesis after Myocardial Infarction.International journal of molecular sciences · 2023Review
- A multi-scale computational model for the passive mechanical behavior of right ventricular myocardium.Journal of the mechanical behavior of biomedical materials · 2023Article
- Multimodal imaging shows fibrosis architecture and action potential dispersion are predictors of arrhythmic risk in spontaneous hypertensive rats.The Journal of physiology · 2022Article
- Confocal microscopy-based estimation of intracellular conductivities in myocardium for modeling of the normal and infarcted heart.Computers in biology and medicine · 2022Article
- It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling.Progress in biophysics and molecular biology · 2022Article
- Long-Term Cultivation of Human Atrial Myocardium.Frontiers in physiology · 2022Article
- Toward cardiac tissue characterization using machine learning and light-scattering spectroscopy.Journal of biomedical optics · 2021Article
- Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging.Scientific reports · 2021Article
- Remodeling of t-system and proteins underlying excitation-contraction coupling in aging versus failing human heart.NPJ aging and mechanisms of disease · 2021Article
- Progressive stretch enhances growth and maturation of 3D stem-cell-derived myocardium.Theranostics · 2021Article
- Etiology-Specific Remodeling in Ventricular Tissue of Heart Failure Patients and Its Implications for Computational Modeling of Electrical Conduction.Frontiers in physiology · 2021Article
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Authors and funding
3 authors.
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Abstract
Microstructural characterization of cardiac tissue and its remodeling in disease is a crucial step in many basic research projects. We present a comprehensive approach for three-dimensional characterization of cardiac tissue at the submicrometer scale. We developed a compression-free mounting method as well as labeling and imaging protocols that facilitate acquisition of three-dimensional image stacks with scanning confocal microscopy. We evaluated the approach with normal and infarcted ventricular tissue. We used the acquired image stacks for segmentation, quantitative analysis and visualization of important tissue components. In contrast to conventional mounting, compression-free mounting preserved cell shapes, capillary lumens and extracellular laminas. Furthermore, the new approach and imaging protocols resulted in high signal-to-noise ratios at depths up to 60 µm. This allowed extensive analyzes revealing major differences in volume fractions and distribution of cardiomyocytes, blood vessels, fibroblasts, myofibroblasts and extracellular space in control vs. infarct border zone. Our results show that the developed approach yields comprehensive data on microstructure of cardiac tissue and its remodeling in disease. In contrast to other approaches, it allows quantitative assessment of all major tissue components. Furthermore, we suggest that the approach will provide important data for physiological models of cardiac tissue at the submicrometer scale.
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