ArticleScientific reports2015
Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity.PLoS pathogens · 2024Article
- Regulation of Tau Expression in Superior Cervical Ganglion (SCG) Neurons In Vivo and In Vitro.Cells · 2023Article
- The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Reversible binding of divalent cations toFrontiers in molecular biosciences · 2023Review
- A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron culturesFrontiers in cellular neuroscience · 2023Article
- A functional circuit formed by the autonomic nerves and myofibroblasts controls mammalian alveolar formation for gas exchange.Developmental cell · 2022Article
- Genome-wide analysis identified novel susceptible genes of restless legs syndrome in migraineurs.The journal of headache and pain · 2022Article
- Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons.EMBO reports · 2022Article
- Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
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4 authors.
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Abstract
Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.
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