ArticlePloS one2015
Ixodes ricinus and Its Endosymbiont Midichloria mitochondrii: A Comparative Proteomic Analysis of Salivary Glands and Ovaries.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins ofLife (Basel, Switzerland) · 2025Article
- Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae).Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025Article
- Could the Oxidation of α1-Antitrypsin Prevent the Binding of Human Neutrophil Elastase in COVID-19 Patients?International journal of molecular sciences · 2023Article
- A high-quality Ixodes scapularis genome advances tick science.Nature genetics · 2023Article
- The Biological and Ecological Features of Northbound Migratory Birds, Ticks, and Tick-Borne Microorganisms in the African-Western Palearctic.Microorganisms · 2023Review
- Lock and Key: WhyPathogens (Basel, Switzerland) · 2022Article
- Co-Occurrence ofMicroorganisms · 2022Article
- Investigating the Link between Alpha-1 Antitrypsin and Human Neutrophil Elastase in Bronchoalveolar Lavage Fluid of COVID-19 Patients.Current issues in molecular biology · 2022Article
- GPR120 prevents colorectal adenocarcinoma progression by sustaining the mucosal barrier integrity.Scientific reports · 2022Article
- What do we know about the microbiome ofFrontiers in cellular and infection microbiology · 2022Review
- Unbiased Characterization of the Microbiome and Virome of Questing Ticks.Frontiers in microbiology · 2021Article
- Interactions Between Ticks and Lyme Disease Spirochetes.Current issues in molecular biology · 2021Article
- Biotic Factors Influence Microbiota of Nymph Ticks from Vegetation in Sydney, Australia.Pathogens (Basel, Switzerland) · 2020Article
- The scale affects our view on the identification and distribution of microbial communities in ticks.Parasites & vectors · 2020Review
- Salivary gland proteome analysis of developing adult female Haemaphysalis longicornis ticks: molecular motor and TCA cycle-related proteins play an important role throughout development.Parasites & vectors · 2019Article
- Morph-specific protein patterns in the femoral gland secretions of a colour polymorphic lizard.Scientific reports · 2019Article
- A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients.High-throughput · 2019Article
- Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Proteome science · 2018Article
- Molecular detection of pathogens in ticks infesting cattle in Nampula province, Mozambique.Experimental & applied acarology · 2017Article
- The Tick Microbiome: Why Non-pathogenic Microorganisms Matter in Tick Biology and Pathogen Transmission.Frontiers in cellular and infection microbiology · 2017Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hard ticks are hematophagous arthropods that act as vectors of numerous pathogenic microorganisms of high relevance in human and veterinary medicine. Ixodes ricinus is one of the most important tick species in Europe, due to its role of vector of pathogenic bacteria such as Borrelia burgdorferi and Anaplasma phagocytophilum, of viruses such as tick borne encephalitis virus and of protozoans as Babesia spp. In addition to these pathogens, I. ricinus harbors a symbiotic bacterium, Midichloria mitochondrii. This is the dominant bacteria associated to I. ricinus, but its biological role is not yet understood. Most M. mitochondrii symbionts are localized in the tick ovaries, and they are transmitted to the progeny. M. mitochondrii bacteria have however also been detected in the salivary glands and saliva of I. ricinus, as well as in the blood of vertebrate hosts of the tick, prompting the hypothesis of an infectious role of this bacterium. To investigate, from a proteomic point of view, the tick I. ricinus and its symbiont, we generated the protein profile of the ovary tissue (OT) and of salivary glands (SG) of adult females of this tick species. To compare the OT and SG profiles, 2-DE profiling followed by LC-MS/MS protein identification were performed. We detected 21 spots showing significant differences in the relative abundance between the OT and SG, ten of which showed 4- to 18-fold increase/decrease in density. This work allowed to establish a method to characterize the proteome of I. ricinus, and to detect multiple proteins that exhibit a differential expression profile in OT and SG. Additionally, we were able to use an immunoproteomic approach to detect a protein from the symbiont. Finally, the method here developed will pave the way for future studies on the proteomics of I. ricinus, with the goals of better understanding the biology of this vector and of its symbiont M. mitochondrii.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.