ArticleParasites & vectors2015
Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Genetic control of malaria transmission: a salivary gland-centric perspective.Current opinion in insect science · 2026Review
- High-resolution proteomics unveils salivary gland disruption and saliva-hemolymph protein exchange in Plasmodium-infected mosquitoes.Nature communications · 2025Article
- Avian malaria and the overlooked metabolic pathways underlying mosquitoRoyal Society open science · 2025Article
- Effects of Plasmodium berghei infection on the expression of salivary gland immune-related genes in the Anopheles stephensi mosquito.Malaria journal · 2025Article
- Advances in the dissection of Anopheles-Plasmodium interactions.PLoS pathogens · 2025Review
- Identification of long noncoding RNAs (lncRNAs) and co-transcriptional analysis of mRNAs and lncRNAs in transcriptomes ofFrontiers in RNA research · 2025Article
- Post-Translational Modifications of Proteins of Malaria Parasites during the Life Cycle.International journal of molecular sciences · 2024Review
- Peptides with Antimicrobial Activity in the Saliva of the Malaria VectorInternational journal of molecular sciences · 2024Article
- Neofunctionalization driven by positive selection led to the retention of the loqs2 gene encoding an Aedes specific dsRNA binding protein.BMC biology · 2024Article
- Transcriptome profiles of Anopheles gambiae harboring natural low-level Plasmodium infection reveal adaptive advantages for the mosquito.Scientific reports · 2021Article
- Effects of Local and Systemic Immune Challenges on the Expression of Selected Salivary Genes in the Malaria MosquitoPathogens (Basel, Switzerland) · 2021Article
- The regulatory genome of the malaria vectorNAR genomics and bioinformatics · 2021Article
- Dengue infection modulates locomotion and host seeking in Aedes aegypti.PLoS neglected tropical diseases · 2020Article
- Glucose transporter GLUT1 influences Plasmodium berghei infection in Anopheles stephensi.Parasites & vectors · 2020Article
- Antibody Responses AgainstFrontiers in cellular and infection microbiology · 2020Article
- Systems Biology-Based Investigation of Host-Plasmodium Interactions.Trends in parasitology · 2018Review
- Serine protease-related proteins in the malaria mosquito, Anopheles gambiae.Insect biochemistry and molecular biology · 2017Article
- Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands.Scientific reports · 2017Article
- Reduction of Mosquito Survival in Mice Vaccinated withBioMed research international · 2017Article
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria is a devastating infectious disease caused by Plasmodium parasites transmitted through the bites of infected Anopheles mosquitoes. Salivary glands are the only mosquito tissue invaded by Plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of Anopheles sialome highly relevant.
methodsRNA-sequencing was used to compare differential gene expression in salivary glands of uninfected and Plasmodium berghei-infected Anopheles coluzzii mosquitoes. RNA-seq results were validated by quantitative RT-PCR. The transmembrane glucose transporter gene AGAP007752 was selected for functional analysis by RNA interference. The effect of gene silencing on infection level was evaluated. The putative function and tertiary structure of the protein was assessed.
resultsRNA-seq data showed that 2588 genes were differentially expressed in mosquitoes salivary glands in response to P. berghei infection, being 1578 upregulated and 1010 downregulated. Metabolism, Immunity, Replication/Transcription/Translation, Proteolysis and Transport were the mosquito gene functional classes more affected by parasite infection. Endopeptidase coding genes were the most abundant within the differentially expressed genes in infected salivary glands (P < 0.001). Based on its putative function and expression level, the transmembrane glucose transporter gene, AGAP007752, was selected for functional analysis by RNA interference. The results demonstrated that the number of sporozoites was 44.3% lower in mosquitoes fed on infected mice after AGAPP007752 gene knockdown when compared to control (P < 0.01).
conclusionsOur hypothesis is that the protein encoded by the gene AGAPP007752 may play a role on An. coluzzii salivary glands infection by Plasmodium parasite, working as a sporozoite receptor and/or promoting a favorable environment for the capacity of sporozoites.
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