Evidence map›Paper›PMID 26395841›Full record

ArticlePlant physiology2015

A Grapevine Anthocyanin Acyltransferase, Transcriptionally Regulated by VvMYBA, Can Produce Most Acylated Anthocyanins Present in Grape Skins.

Amy R Rinaldo, Erika Cavallini, Yong Jia, Sarah M A Moss, Debra A J McDavid, Lauren C Hooper, Simon P Robinson, Giovanni B Tornielli, Sara Zenoni, Christopher M Ford and 2 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed, 1 pooled it
18.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed, 1 synthesis or guideline pooled it, 165 citations in OpenAlex.

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22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Amy R RinaldoCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0001-6450-2657
Erika CavalliniCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).
Yong JiaCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0002-0394-3966
Sarah M A MossCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0002-0060-5486
Debra A J McDavidCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).
Lauren C HooperCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).
Simon P RobinsonCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0003-4812-6094
Giovanni B TornielliCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0001-5027-0269
Sara ZenoniCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0002-0496-8161
Christopher M FordCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).
Paul K BossCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.).ORCID http://orcid.org/0000-0003-0356-9342
Amanda R WalkerCommonwealth Scientific and Industrial Research Organization-Agriculture, Wine Innovation West, Hartley Grove, South Australia 5064, Australia (A.R.R., S.M.A.M., D.A.J.M., L.C.H., S.P.R., P.K.B., A.R.W.);School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, South Australia 5005, Australia (A.R.R., Y.J., S.M.A.M., C.M.F.); andDepartment of Biotechnology, University of Verona, 37134 Verona, Italy (E.C., G.B.T., S.Z.) mandy.walker@csiro.au.ORCID http://orcid.org/0000-0002-7596-8484
University of Adelaide · AUUniversity of Verona · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anthocyanins are flavonoid compounds responsible for red/purple colors in the leaves, fruit, and flowers of many plant species. They are produced through a multistep pathway that is controlled by MYB transcription factors. VvMYBA1 and VvMYBA2 activate anthocyanin biosynthesis in grapevine (Vitis vinifera) and are nonfunctional in white grapevine cultivars. In this study, transgenic grapevines with altered VvMYBA gene expression were developed, and transcript analysis was carried out on berries using a microarray technique. The results showed that VvMYBA is a positive regulator of the later stages of anthocyanin synthesis, modification, and transport in cv Shiraz. One up-regulated gene, ANTHOCYANIN 3-O-GLUCOSIDE-6″-O-ACYLTRANSFERASE (Vv3AT), encodes a BAHD acyltransferase protein (named after the first letter of the first four characterized proteins: BEAT [for acetyl CoA:benzylalcohol acetyltransferase], AHCT [for anthocyanin O-hydroxycinnamoyltransferase], HCBT [for anthranilate N-hydroxycinnamoyl/benzoyltransferase], and DAT [for deacetylvindoline 4-O-acetyltransferase]), belonging to a clade separate from most anthocyanin acyltransferases. Functional studies (in planta and in vitro) show that Vv3AT has a broad anthocyanin substrate specificity and can also utilize both aliphatic and aromatic acyl donors, a novel activity for this enzyme family found in nature. In cv Pinot Noir, a red-berried grapevine mutant lacking acylated anthocyanins, Vv3AT contains a nonsense mutation encoding a truncated protein that lacks two motifs required for BAHD protein activity. Promoter activation assays confirm that Vv3AT transcription is activated by VvMYBA1, which adds to the current understanding of the regulation of the BAHD gene family. The flexibility of Vv3AT to use both classes of acyl donors will be useful in the engineering of anthocyanins in planta or in vitro.

Indexed as

Gene Expression Regulation, PlantAcylationAcyltransferasesAnthocyaninsFlavonoidsPlant ProteinsPlants, Genetically ModifiedTranscription FactorsVitisAcyltransferasesAnthocyaninsanthranilate N-hydroxycinnamoyl benzoyltransferaseFlavonoidshydroxycinnamoyl-CoA - anthocyanin 3-O-glucoside-6''-O-acyltransferasePlant ProteinsTranscription Factors

Identifiers

PMID26395841
PMCPMC4634099
OpenAlexW1927521981

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.