ArticleDevelopment (Cambridge, England)2015
ChtVis-Tomato, a genetic reporter for in vivo visualization of chitin deposition in Drosophila.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Distinct cellular and molecular mechanisms contribute to the specificity of the two Drosophila melanogaster chitin synthases in chitin deposition.PLoS genetics · 2025Article
- Apical cell expansion maintained by Dusky-like establishes a scaffold for corneal lens morphogenesis.Science advances · 2024Article
- Article
- Article
- Recombinant protein linker production as a basis for non-invasive determination of single-cell yeast age in heterogeneous yeast populations.RSC advances · 2021Article
- Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper.Journal of insect science (Online) · 2019Article
- An Ichor-dependent apical extracellular matrix regulates seamless tube shape and integrity.PLoS genetics · 2018Article
- Article
- The Gene Expression Program for the Formation of Wing Cuticle in Drosophila.PLoS genetics · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Chitin is a polymer of N-acetylglucosamine that is abundant and widely found in the biological world. It is an important constituent of the cuticular exoskeleton that plays a key role in the insect life cycle. To date, the study of chitin deposition during cuticle formation has been limited by the lack of a method to detect it in living organisms. To overcome this limitation, we have developed ChtVis-Tomato, an in vivo reporter for chitin in Drosophila. ChtVis-Tomato encodes a fusion protein that contains an apical secretion signal, a chitin-binding domain (CBD), a fluorescent protein and a cleavage site to release it from the plasma membrane. The chitin reporter allowed us to study chitin deposition in time lapse experiments and by using it we have identified unexpected deposits of chitin fibers in Drosophila pupae. ChtVis-Tomato should facilitate future studies on chitin in Drosophila and other insects.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.