ArticleDevelopment (Cambridge, England)2015
Polarized Rac-dependent protrusions drive epithelial intercalation in the embryonic epidermis of C. elegans.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Reciprocal inhibition of Wnt signaling pathways pattern the interconnection of epithelial tubules in the regenerating zebrafish kidney.Development (Cambridge, England) · 2026Article
- A dystroglycan-laminin-integrin axis coordinates cell shape remodeling in the developing Drosophila retina.PLoS biology · 2024Article
- The Rac pathway prevents cell fragmentation in a nonprotrusively migrating leader cell during C. elegans gonad organogenesis.Current biology : CB · 2024Article
- TIAM-1 regulates polarized protrusions during dorsal intercalation in the Caenorhabditis elegans embryo through both its GEF and N-terminal domains.Journal of cell science · 2024Article
- Getting there in one piece: The Rac pathway prevents cell fragmentation in a nonprotrusively migrating leader cell during organogenesis.bioRxiv : the preprint server for biology · 2023Article
- Imaging Epidermal Cell Rearrangement in the C. elegans Embryo.Methods in molecular biology (Clifton, N.J.) · 2022Review
- The Lamellipodin homologue MIG-10 is not essential for dorsal intercalation in the embryonic epidermis of themicroPublication biology · 2022Article
- Won't You be My Neighbor: How Epithelial Cells Connect Together to Build Global Tissue Polarity.Frontiers in cell and developmental biology · 2022Review
- Functions of Arp2/3 Complex in the Dynamics of Epithelial Tissues.Frontiers in cell and developmental biology · 2022Review
- Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells.PLoS genetics · 2021Article
- Engineering threshold-based selection systems.G3 (Bethesda, Md.) · 2021Article
- Twinfilin1 controls lamellipodial protrusive activity and actin turnover during vertebrate gastrulation.Journal of cell science · 2021Article
- A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants.G3 (Bethesda, Md.) · 2021Article
- Programmed and self-organized flow of information during morphogenesis.Nature reviews. Molecular cell biology · 2021Review
- Trio family proteins as regulators of cell migration and morphogenesis in development and disease - mechanisms and cellular contexts.Journal of cell science · 2021Review
- Epithelial morphogenesis, tubulogenesis and forces in organogenesis.Current topics in developmental biology · 2021Review
- RhoGAP RGA-8 supports morphogenesis inBiology open · 2020Article
- Cell intercalation in a simple epithelium.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Orchestrating morphogenesis: building the body plan by cell shape changes and movements.Development (Cambridge, England) · 2020Review
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3 authors at 2 institutions in 1 country.
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Abstract
Cell intercalation is a fundamental, coordinated cell rearrangement process that shapes tissues throughout animal development. Studies of intercalation within epithelia have focused almost exclusively on the localized constriction of specific apical junctions. Another widely deployed yet poorly understood alternative mechanism of epithelial intercalation relies on basolateral protrusive activity. Using the dorsal embryonic epidermis of Caenorhabditis elegans, we have investigated this alternative mechanism using high-resolution live cell microscopy and genetic analysis. We find that as dorsal epidermal cells migrate past one another they produce F-actin-rich protrusions polarized at their extending (medial) edges. These protrusions are controlled by the C. elegans Rac and RhoG orthologs CED-10 and MIG-2, which function redundantly to polarize actin polymerization upstream of the WAVE complex and WASP, respectively. We also identify UNC-73, the C. elegans ortholog of Trio, as a guanine nucleotide exchange factor (GEF) upstream of both CED-10 and MIG-2. Further, we identify a novel polarizing cue, CRML-1, which is the ortholog of human capping Arp2/3 myosin I linker (CARMIL), that localizes to the nonprotrusive lateral edges of dorsal cells. CRML-1 genetically suppresses UNC-73 function and, indirectly, actin polymerization. This network identifies a novel, molecularly conserved cassette that regulates epithelial intercalation via basolateral protrusive activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.