ArticleTransgenic research2015
Perinatal induction of Cre recombination with tamoxifen.
Article in Transgenic research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 60 citations in OpenAlex.
- A new conditional mouse model for Cre-mediated restoration ofbioRxiv : the preprint server for biology · 2026Article
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- Evolving stem cell fate capacities and transcriptional priming in the developing olfactory epithelium.bioRxiv : the preprint server for biology · 2026Article
- Postnatal relevance of HOXA5 transcription factor in cerebellum-associated behaviors and disorders.Behavioral and brain functions : BBF · 2026Article
- Defective ventral neurogenesis due to midfetal Chd8 mutation drives autistic-like behavior in mice.Nature communications · 2026Article
- GPR124 regulates hyaloid blood vessel regression and is associated with endothelial-mesenchymal transition.Scientific reports · 2026Article
- Vascularization of neonatal liver lobules presages adult liver size.Nature communications · 2025Article
- Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.Developmental cell · 2025Article
- Development of a Hepatocyte-Specific Temporal Genetic Mouse Model Using Albumin Promoter-Driven FlpER2 Expression.Genesis (New York, N.Y. : 2000) · 2025Article
- Article
- A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†.Biology of reproduction · 2025Article
- Primary Cell Cultures in Neurobiology: Optimized Protocol for Culture of Mouse Fetal Hindbrain Neurons.Cells · 2025Article
- Article
- Intestinal gluconeogenesis controls the neonatal development of hypothalamic feeding circuits.Molecular metabolism · 2024Article
- Dynamic Tracking of Native Precursors in Adult Mice.bioRxiv : the preprint server for biology · 2024Article
- Interplay between Pitx2 and Pax7 temporally governs specification of extraocular muscle stem cells.PLoS genetics · 2024Article
- Overexpression of wild-type HRAS drives non-alcoholic steatohepatitis to hepatocellular carcinoma in mice.Zoological research · 2024Article
- Villus myofibroblasts are developmental and adult progenitors of mammalian gut lymphatic musculature.Developmental cell · 2024Article
- Review
- N-cadherin and β1 integrin coordinately regulate growth plate cartilage architecture.Molecular biology of the cell · 2024Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or early postnatal stages is still difficult to achieve. In this context, using the ubiquitous CMV-CreER(T2) transgenic mice, we have tested and validated two procedures to achieve recombination just before and just after birth. The efficiency of recombination was evaluated in the brain, which is known to be more problematic to target. For the late gestation treatment with tamoxifen, different protocols of complementary administration of progesterone and estrogen were tested. However, delayed delivery and/or mortality of pups due to difficult delivery were always observed. To circumvent this problem, pups were collected from tamoxifen-treated pregnant dams by caesarian section at E18.5 and given to foster mothers. For postnatal treatment, different dosages of tamoxifen were administered by intragastric injection to the pups during 3 or 4 days after birth. The efficiency of these treatments was analyzed at P7 using a transgenic reporter line. They were also validated with the Hoxa5 conditional allele. In conclusion, we have developed efficient procedures that allow achieving efficient recombination of floxed alleles at perinatal stages. These protocols will allow investigating the late/adult functions of many developmental genes, whose characterization has been so far restricted to embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.