Evidence map›Paper›PMID 26395124›Full record

ArticleIn vitro cellular & developmental biology. Animal2016

Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis.

Mohammad Ghasemzadeh-Hasankolaei, Mohammadreza Baghaban Eslaminejad, Mohammadali Sedighi-Gilani

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 2 pooled it
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.

  1. Pooled it
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  7. Comparison of the efficacy of bone morphogenetic protein-4 onResearch in pharmaceutical sciences · 2022
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  17. Amniotic membrane mesenchymal stem cells can differentiate into germ cells in vitro.In vitro cellular & developmental biology. Animal · 2016
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Mohammad Ghasemzadeh-HasankolaeiStem Cell Research Lab, Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Beginning of Babol-Amol Old Highway, P.O. Box: 47135-547, Babol, Iran. ghasemzadeh78@gmail.com.
Mohammadreza Baghaban EslaminejadDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Bani Hashem Sq., Bani Hashem St., Resalat Highway, P.O. Box 19395-4644, Tehran, Iran. bagesla@yahoo.com.
Mohammadali Sedighi-GilaniDepartment of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Royan Institute · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) have the capacity to differentiate into germ cells (GCs). This research, for the first time, has evaluated the fate of in vitro MSC-derived GCs generated by three different induction methods and compared them after transplantation into the testes of rams. Passage-3 ram bone marrow (BM)-MSCs were divided into three treatment groups: (1) 14-d treatment with 10 μM retinoic acid (RA; RA14), (2) 21-d treatment with 10 μM RA (RA21), and (3) 21-d treatment with 10 ng/ml transforming growth factor beta-1 (TGFb1). After confirmation of the existence of germ-like cells in the culture, the treated cells were labeled and transplanted into the testes of ram lambs. After 2 mo, we conducted histological evaluations of the rams' testes. Results showed that in vitro-derived GCs from all treatment groups survived in the testes. Some of these GCs homed at the basement membrane of seminiferous tubules and formed colonies. The homed cells and cell colonies were similar to testicular native spermatogonia and expressed PGP9.5. TGFb1 exhibited the highest efficiency for in vitro production of GCs as well as the highest capability for homing and colony formation in the testes. RA21 was less efficient than TGFb1, particularly in colony formation. RA14 was the weakest group. No further differentiation of the transplanted GCs was observed. From our results, it could be concluded that a 21-d treatment period of BM-MSCs with TGFb1 is the most efficient method for in vitro generation of spermatogonia-like cells that survive, home, and form colonies in the testes.

Indexed as

Mesenchymal Stem Cell TransplantationAnimalsBone Marrow CellsCell DifferentiationGene Expression Regulation, DevelopmentalGerm CellsHematopoietic Stem CellsMaleMesenchymal Stem CellsSeminiferous TubulesSheep, DomesticSpermatogenesisSpermatogoniaTestisTransforming Growth Factor beta1Transforming Growth Factor beta1FateGerm cellsIn vitroMesenchymal stem cellsRamTestisTransplantation

Identifiers

PMID26395124
OpenAlexW1721288641

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.