ArticlePlant molecular biology2015
Defining multiple, distinct, and shared spatiotemporal patterns of DNA replication and endoreduplication from 3D image analysis of developing maize (Zea mays L.) root tip nuclei.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Replication timing uncovers a two-compartment nuclear architecture of interphase euchromatin.The Plant cell · 2026Article
- KMT2C/KMT2D-dependent H3K4me1 mediates changes in DNA replication timing and origin activity during a cell fate transition.Cell reports · 2025Article
- MLL3/MLL4 enzymatic activity shapes DNA replication timing.bioRxiv : the preprint server for biology · 2023Article
- Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition.Nature communications · 2023Article
- From the archives: Polar auxin transport in nodule development, DNA replication timing, and developmentally light-regulated genes.The Plant cell · 2022Article
- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Plant 3D Chromatin Organization: Important Insights from Chromosome Conformation Capture Analyses of the Last 10 Years.Plant & cell physiology · 2021Review
- The supernumerary B chromosome of maize: drive and genomic conflict.Open biology · 2021Article
- The 3D Genome: From Structure to Function.International journal of molecular sciences · 2021Review
- Sequence of the supernumerary B chromosome of maize provides insight into its drive mechanism and evolution.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots.PLoS genetics · 2020Article
- Arabidopsis DNA Replication Initiates in Intergenic, AT-Rich Open Chromatin.Plant physiology · 2020Article
- Impact of Mutagens on DNA Replication in Barley Chromosomes.International journal of molecular sciences · 2018Article
- Genome-Wide Analysis of the Arabidopsis Replication Timing Program.Plant physiology · 2018Article
- Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals.Plant physiology · 2018Review
- Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (The Plant cell · 2017Article
- Repliscan: a tool for classifying replication timing regions.BMC bioinformatics · 2017Article
- Take a look at plant DNA replication: Recent insights and new questions.Plant signaling & behavior · 2017Article
- Highlight on the dynamic organization of the nucleus.Nucleus (Austin, Tex.) · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatiotemporal patterns of DNA replication have been described for yeast and many types of cultured animal cells, frequently after cell cycle arrest to aid in synchronization. However, patterns of DNA replication in nuclei from plants or naturally developing organs remain largely uncharacterized. Here we report findings from 3D quantitative analysis of DNA replication and endoreduplication in nuclei from pulse-labeled developing maize root tips. In both early and middle S phase nuclei, flow-sorted on the basis of DNA content, replicative labeling was widely distributed across euchromatic regions of the nucleoplasm. We did not observe the perinuclear or perinucleolar replicative labeling patterns characteristic of middle S phase in mammals. Instead, the early versus middle S phase patterns in maize could be distinguished cytologically by correlating two quantitative, continuous variables, replicative labeling and DAPI staining. Early S nuclei exhibited widely distributed euchromatic labeling preferentially localized to regions with weak DAPI signals. Middle S nuclei also exhibited widely distributed euchromatic labeling, but the label was preferentially localized to regions with strong DAPI signals. Highly condensed heterochromatin, including knobs, replicated during late S phase as previously reported. Similar spatiotemporal replication patterns were observed for both mitotic and endocycling maize nuclei. These results revealed that maize euchromatin exists as an intermingled mixture of two components distinguished by their condensation state and replication timing. These different patterns might reflect a previously described genome organization pattern, with "gene islands" mostly replicating during early S phase followed by most of the intergenic repetitive regions replicating during middle S phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.