ArticleJournal of leukocyte biology2016
TCRβ repertoire of CD4+ and CD8+ T cells is distinct in richness, distribution, and CDR3 amino acid composition.
Article in Journal of leukocyte biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 53 citations in OpenAlex.
- The Role of the T Cell Receptor Sequence in Shaping T Cell Functional Fate.Immunological reviews · 2026Review
- CD8⁺ T cell heterogeneity in aging: insights from single-cell profiling.Immunity & ageing : I & A · 2025Review
- A pan-disease and population-level single-cell TCRαβ repertoire reference.Cell discovery · 2025Article
- General and individualized changes in T cell immunity during aging.Journal of immunology (Baltimore, Md. : 1950) · 2025Review
- Age-Related Dynamics and Spectral Characteristics of the TCRβ Repertoire in Healthy Children: Implications for Immune Aging.Aging cell · 2025Article
- The T cell receptor sequence influences the likelihood of T cell memory formation.Cell reports · 2025Article
- ATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs.Nature communications · 2025Article
- A fundamental relationship between TCR diversity, repertoire size and systemic clonal expansion: insights from 30,000 TCRFrontiers in immunology · 2025Article
- Minimally expanded breast cancer tumor-infiltrating-lymphocytes provide guidance for therapeutic selection.Frontiers in immunology · 2025Article
- tcrBLOSUM: an amino acid substitution matrix for sensitive alignment of distant epitope-specific TCRs.Briefings in bioinformatics · 2024Article
- Innate-like T cell subset commitment in the murine thymus is independent of TCR characteristics and occurs during proliferation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- SARS-CoV-2 infection establishes a stable and age-independent CD8Nature communications · 2023Article
- Human thymic putative CD8αα precursors exhibit a biased TCR repertoire in single cell AIRR-seq.Scientific reports · 2023Article
- Numbers and odds: TCR repertoire size and its age changes impacting on T cell functions.Seminars in immunology · 2023Review
- The thymoproteasome in shaping the CD8Current opinion in immunology · 2023Review
- Evolutionary trade-offs constraining the MHC gene expansion: beyond simple TCR depletion model.Frontiers in immunology · 2023Article
- Dynamic kernel matching for non-conforming data: A case study of T cell receptor datasets.PloS one · 2023Article
- Sex- and age-specific aspects of human peripheral T-cell dynamics.Frontiers in immunology · 2023Article
- Analysis of tumor infiltrating CD4+ and CD8+ CDR3 sequences reveals shared features putatively associated to the anti-tumor immune response.Frontiers in immunology · 2023Article
- Longitudinal analysis reveals age-related changes in the T cell receptor repertoire of human T cell subsets.The Journal of clinical investigation · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
The TCR repertoire serves as a reservoir of TCRs for recognizing all potential pathogens. Two major types of T cells, CD4(+) and CD8(+), that use the same genetic elements and process to generate a functional TCR differ in their recognition of peptide bound to MHC class II and I, respectively. However, it is currently unclear to what extent the TCR repertoire of CD4(+) and CD8(+) T cells is different. Here, we report a comparative analysis of the TCRβ repertoires of CD4(+) and CD8(+) T cells by use of a 5' rapid amplification of cDNA ends-PCR-sequencing method. We found that TCRβ richness of CD4(+) T cells ranges from 1.2 to 9.8 × 10(4) and is approximately 5 times greater, on average, than that of CD8(+) T cells in each study subject. Furthermore, there was little overlap in TCRβ sequences between CD4(+) (0.3%) and CD8(+) (1.3%) T cells. Further analysis showed that CD4(+) and CD8(+) T cells exhibited distinct preferences for certain amino acids in the CDR3, and this was confirmed further by a support vector machine classifier, suggesting that there are distinct and discernible differences between TCRβ CDR3 in CD4(+) and CD8(+) T cells. Finally, we identified 5-12% of the unique TCRβs that share an identical CDR3 with different variable genes. Together, our findings reveal the distinct features of the TCRβ repertoire between CD4(+) and CD8(+) T cells and could potentially be used to evaluate the competency of T cell immunity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.