ArticleParasites & vectors2015
Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 113 citations in OpenAlex.
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- Vaccination-Route-Dependent Adjuvanticity of Antigen-Carrying Nanoparticles for Enhanced Vaccine Efficacy.Vaccines · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis.Veterinary research · 2024Article
- PLGA-EncapsulatedVaccines · 2023Article
- Identification of antigens in the Trichinella spiralis extracellular vesicles for serological detection of early stage infection in swine.Parasites & vectors · 2023Article
- Metabolomics of Type 2 Diabetes Mellitus in Sprague Dawley Rats-In Search of Potential Metabolic Biomarkers.International journal of molecular sciences · 2023Article
- Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Scheme of Effective Epidemiological Investigations inFoods (Basel, Switzerland) · 2023Article
- Untargeted serum metabolomics analysis of Trichinella spiralis-infected mouse.PLoS neglected tropical diseases · 2023Article
- Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge.Parasites & vectors · 2022Article
- Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice.PLoS neglected tropical diseases · 2022Article
- Characterization of a novel pyruvate kinase from Trichinella spiralis and its participation in sugar metabolism, larval molting and development.PLoS neglected tropical diseases · 2022Article
- Molecular characterization and determination of the biochemical properties of cathepsin L of Trichinella spiralis.Veterinary research · 2022Article
- Lateral flow immunoassay strips based on europium(III) chelate microparticle for the rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2022Article
- Oral vaccination with recombinant Lactobacillus plantarum encoding Trichinella spiralis inorganic pyrophosphatase elicited a protective immunity in BALB/c mice.PLoS neglected tropical diseases · 2021Article
- Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity.Veterinary research · 2021Article
- Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development.Pathogens (Basel, Switzerland) · 2021Review
- Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs.Veterinary research · 2021Article
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9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe excretory-secretory (ES) antigens of Trichinella spiralis muscle larvae (ML) are the most commonly used diagnostic antigens for trichinellosis. Their main disadvantage for the detection of anti-Trichinella IgG is false-negative results during the early stage of infection. Additionally, there is an obvious window between clinical symptoms and positive serology.
methodsELISA with adult worm (AW) ES antigens was used to detect anti-Trichinella IgG in the sera of experimentally infected mice and patients with trichinellosis. The sensitivity and specificity were compared with ELISAs with AW crude antigens and ML ES antigens.
resultsIn mice infected with 100 ML, anti-Trichinella IgG were first detected by ELISA with the AW ES antigens, crude antigens and ML ES antigens 8, 12 and 12 days post-infection (dpi), respectively. In mice infected with 500 ML, specific antibodies were first detected by ELISA with the three antigen preparations at 10, 8 and 10 dpi, respectively. The sensitivity of the ELISA with the three antigen preparations for the detection of sera from patients with trichinellosis at 35 dpi was 100%. However, when the patients' sera were collected at 19 dpi, the sensitivities of the ELISAs with the three antigen preparations were 100% (20/20), 100% (20/20) and 75% (15/20), respectively (P < 0.05). The specificities of the ELISAs with the three antigen preparations were 98.11, 95.60 and 89.31%, respectively (P < 0.05).
conclusionsThe sensitivity and specificity of the T. spiralis AW ES antigens were superior to those of the AW crude antigens and ML ES antigens. Thus, the AW ES antigens might serve as potential antigens for the early and specific serodiagnosis of trichinellosis.
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