ArticlePLoS neglected tropical diseases2015
Discrimination between E. granulosus sensu stricto, E. multilocularis and E. shiquicus Using a Multiplex PCR Assay.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Establishment of a secondary infection laboratory model ofParasitology · 2023Article
- Brief report prevalence of Echinococcus species in wild foxes in parts of Qinghai Province, China.Veterinary research communications · 2023Article
- EmergingTransboundary and emerging diseases · 2023Article
- Prevalence of Intestinal Parasites in Dog Faecal Samples from Public Environments in Qinghai Province, China.Pathogens (Basel, Switzerland) · 2022Article
- Diagnostic tools for the detection of taeniid eggs in different environmental matrices: A systematic review.Food and waterborne parasitology · 2022Article
- Prevalence of Echinococcus Species in Wild Foxes and Stray Dogs in Qinghai Province, China.The American journal of tropical medicine and hygiene · 2021Article
- Identification of Different Extracellular Vesicles in the Hydatid Fluid ofFrontiers in immunology · 2021Article
- The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model.Scientific reports · 2019Article
- A multiplex PCR assay for the simultaneous detection of Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum infections.BMC infectious diseases · 2019Article
- Genetic characterization ofParasitology · 2019Article
- Cystic echinococcosis in Nigeria: first insight into the genotypes of Echinococcus granulosus in animals.Parasites & vectors · 2019Article
- A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto, Echinococcus multilocularis and Echinococcus canadensis in China.Infectious diseases of poverty · 2019Article
- Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1-G3 genotype.Molecular biology reports · 2019Article
- Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis.Infectious diseases of poverty · 2018Article
- A novel PCR-RFLP assay for molecular characterization of Echinococcus granulosus sensu lato and closely related species in developing countries.Parasitology research · 2016Article
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13 authors.
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Abstract
backgroundInfections of Echinococcus granulosus sensu stricto (s.s), E. multilocularis and E. shiquicus are commonly found co-endemic on the Qinghai-Tibet plateau, China, and an efficient tool is needed to facilitate the detection of infected hosts and for species identification. METHODOLOGY/PRINCIPAL
findingsA single-tube multiplex PCR assay was established to differentiate the Echinococcus species responsible for infections in intermediate and definitive hosts. Primers specific for E. granulosus, E. multilocularis and E. shiquicus were designed based on sequences of the mitochondrial NADH dehydrogenase subunit 1 (nad1), NADH dehydrogenase subunit 5 (nad5) and cytochrome c oxidase subunit 1 (cox1) genes, respectively. This multiplex PCR accurately detected Echinococcus DNA without generating nonspecific reaction products. PCR products were of the expected sizes of 219 (nad1), 584 (nad5) and 471 (cox1) bp. Furthermore, the multiplex PCR enabled diagnosis of multiple infections using DNA of protoscoleces and copro-DNA extracted from fecal samples of canine hosts. Specificity of the multiplex PCR was 100% when evaluated using DNA isolated from other cestodes. Sensitivity thresholds were determined for DNA from protoscoleces and from worm eggs, and were calculated as 20 pg of DNA for E. granulosus and E. shiquicus, 10 pg of DNA for E. multilocularis, 2 eggs for E. granulosus, and 1 egg for E. multilocularis. Positive results with copro-DNA could be obtained at day 17 and day 26 after experimental infection of dogs with larval E. multilocularis and E. granulosus, respectively. CONCLUSIONS/SIGNIFICANCE: The multiplex PCR developed in this study is an efficient tool for discriminating E. granulosus, E. multilocularis and E. shiquicus from each other and from other taeniid cestodes. It can be used for the detection of canids infected with E. granulosus s.s. and E. multilocularis using feces collected from these definitive hosts. It can also be used for the identification of the Echinococcus metacestode larva in intermediate hosts, a stage that often cannot be identified to species on visual inspection.
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