ArticlePLoS genetics2015
Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- A molecular brake that modulates spliceosome pausing at detained introns contributes to neurodegeneration.Protein & cell · 2023Article
- Phylogenetic Analysis of Spliceosome SF3a2 in Different Plant Species.International journal of molecular sciences · 2023Article
- An Arabidopsis Retention and Splicing complex regulates root and embryo development through pre-mRNA splicing.Plant physiology · 2022Article
- Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes.Cold Spring Harbor perspectives in biology · 2019Review
- Dynamic protein-RNA interactions in mediating splicing catalysis.Nucleic acids research · 2019Article
- Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome.Cold Spring Harbor perspectives in biology · 2019Review
- To Splice or to Transcribe: SKIP-Mediated Environmental Fitness and Development in Plants.Frontiers in plant science · 2019Review
- Multiple RNA-RNA tertiary interactions are dispensable for formation of a functional U2/U6 RNA catalytic core in the spliceosome.Nucleic acids research · 2018Article
- Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex.RNA (New York, N.Y.) · 2018Article
- The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated BGenes & development · 2017Article
- Yeast Prp2 liberates the 5' splice site and the branch site adenosine for catalysis of pre-mRNA splicing.RNA (New York, N.Y.) · 2017Article
- The pre-mRNA retention and splicing complex controls expression of the Mediator subunit Med20.RNA biology · 2017Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly.RNA (New York, N.Y.) · 2017Article
- Genetics and biochemistry remain essential in the structural era of the spliceosome.Methods (San Diego, Calif.) · 2017Review
- Structure of a pre-catalytic spliceosome.Nature · 2017Article
- Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain.RNA (New York, N.Y.) · 2017Article
- SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.Nucleic acids research · 2017Article
- Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2.Nucleic acids research · 2017Article
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about contacts in the spliceosome between proteins and intron nucleotides surrounding the pre-mRNA branch-site and their dynamics during splicing. We investigated protein-pre-mRNA interactions by UV-induced crosslinking of purified yeast B(act) spliceosomes formed on site-specifically labeled pre-mRNA, and analyzed their changes after conversion to catalytically-activated B* and step 1 C complexes, using a purified splicing system. Contacts between nucleotides upstream and downstream of the branch-site and the U2 SF3a/b proteins Prp9, Prp11, Hsh49, Cus1 and Hsh155 were detected, demonstrating that these interactions are evolutionarily conserved. The RES proteins Pml1 and Bud13 were shown to contact the intron downstream of the branch-site. A comparison of the B(act) crosslinking pattern versus that of B* and C complexes revealed that U2 and RES protein interactions with the intron are dynamic. Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed. Cwc25's step 1 promoting activity was not dependent on its interaction with pre-mRNA, indicating it acts via protein-protein interactions. These studies provide important insights into the spliceosome's protein-pre-mRNA network and reveal novel RNP remodeling events during the catalytic activation of the spliceosome and step 1 of splicing.
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