ArticleeLife2015
A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Phenotype-specific muscle proteomic profiling in titinopathies.Acta neuropathologica communications · 2026Article
- UTERINE FIBROIDS.Physiological reviews · 2025Review
- Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution.Synthetic and systems biotechnology · 2025Article
- Deubiquitinases in muscle physiology and disorders.Biochemical Society transactions · 2024Review
- USP18 is an essential regulator of muscle cell differentiation and maturation.Cell death & disease · 2023Article
- The roles of E3 ligases in Hepatocellular carcinoma.American journal of cancer research · 2022Review
- Identification of methylation markers for diagnosis of autism spectrum disorder.Metabolic brain disease · 2022Article
- Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.Frontiers in physiology · 2021Article
- The Effect of Dysfunctional Ubiquitin Enzymes in the Pathogenesis of Most Common Diseases.International journal of molecular sciences · 2020Review
- Roles of the HUWE1 ubiquitin ligase in nervous system development, function and disease.Neural development · 2020Review
- The structure and regulation of the E3 ubiquitin ligase HUWE1 and its biological functions in cancer.Investigational new drugs · 2020Review
- Walk the Line: The Role of Ubiquitin in Regulating Transcription in Myocytes.Physiology (Bethesda, Md.) · 2019Review
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- Transforming Growth Factor β-Induced Proliferative Arrest Mediated by TRIM26-Dependent TAF7 Degradation and Its Antagonism by MYC.Molecular and cellular biology · 2018Article
- Ubiquitylation at the crossroads of development and disease.Nature reviews. Molecular cell biology · 2018Review
- Ubiquitin-Dependent Regulation of Stem Cell Biology.Trends in cell biology · 2017Review
- Elevated TATA-binding protein expression drives vascular endothelial growth factor expression in colon cancer.Oncotarget · 2017Article
- The punctilious RNA polymerase II core promoter.Genes & development · 2017Review
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP protein levels are tightly regulated by the ubiquitin-proteasome system. Using an in vitro ubiquitination assay coupled with biochemical fractionation, we identified Huwe1 as an E3 ligase targeting TBP for K48-linked ubiquitination and proteasome-mediated degradation. Upregulation of Huwe1 expression during myogenesis induces TBP degradation and myotube differentiation. We found that Huwe1 activity on TBP is antagonized by the deubiquitinase USP10, which protects TBP from degradation. Thus, modulating the levels of both Huwe1 and USP10 appears to fine-tune the requisite degradation of TBP during myogenesis. Together, our study unmasks a previously unknown interplay between an E3 ligase and a deubiquitinating enzyme regulating TBP levels during cellular differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.