ArticleVeterinary record open2015
Novel real-time PCR detection assay for Brucella suis.
Article in Veterinary record open, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A critical review of diagnostic methods for camel brucellosis.Veterinary and animal science · 2026Review
- Pathological, microbiological, and molecular characterization of uterine samples in culled sows from farms with a history of reproductive failure in Brazil.Veterinary research communications · 2026Article
- A novel duplex TaqMan qPCR assay for rapid differential diagnosis and co-infection screening of Orf virus and Brucella spp. in goats.Veterinary research communications · 2026Article
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Brucella suis in feral pigs in Australia: what is the risk?Australian veterinary journal · 2025Article
- Comparison of Illumina and Oxford Nanopore Technology for genome analysis of Francisella tularensis, Bacillus anthracis, and Brucella suis.BMC genomics · 2023Article
- Monitoring results of wild boar (Infection ecology & epidemiology · 2020Article
- Seroprevalence and Molecular Identification ofMicroorganisms · 2020Article
- Serological and Molecular Identification ofPathogens (Basel, Switzerland) · 2019Article
- Article
- Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood.Viruses · 2017Article
- Swine brucellosis: current perspectives.Veterinary medicine (Auckland, N.Z.) · 2017Review
- Brucellosis in a refugee who migrated from Syria to Germany and lessons learnt, 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2016Article
- Clinical investigation and management of Brucella suis seropositive dogs: A longitudinal case series.Journal of veterinary internal medicineArticle
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionBrucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). Biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in Europe. Both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. Available serological or PCR based methods lack sensitivity and specificity.
resultsHere a bioinformatics approach was used to identify a B. suis specific 17 bp repeat on chromosome II (BS1330_II0657 locus). This repeat is common for B. suis bv 1 to 4 and was used to develop a TaqMan probe assay. The average PCR efficiency was determined as 95% and the limit of detection as 12,5 fg/µl of DNA, equally to 3.7 bacterial genomes. This assay has the highest sensitivity of all previously described B. suis specific PCR assays, making it possible to detect 3-4 bacterial genomes per 1 µl of sample. The assay was tested 100% specific for B. suis and negative for other Brucella spp. and closely related non-Brucella species.
conclusionsThis novel qPCR assay could become a rapid, inexpensive and reliable screening method for large sample pools of B. suis 1 to 4. This method will be applicable for field samples after validation.
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