Evidence map›Paper›PMID 26392829›Full record

ArticleBiomicrofluidics2015

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis.

Weiran Liu, Weixing Chen, Ran Liu, Yuan Ou, Haoran Liu, Lan Xie, Ying Lu, Caixia Li, Bin Li, Jing Cheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomicrofluidics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Weiran Liu
Weixing Chen
Ran LiuDepartment of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, China.
Yuan OuBeijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science , Beijing 100038, China.
Haoran Liu
Lan Xie
Ying Lu
Caixia LiBeijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science , Beijing 100038, China.
Bin LiFujian Provincial Key Laboratory of Forensic Science and Technology , Forensic Science Division, Department of Fujian Provincial Public Security, Fuzhou 350003, China.
Jing Cheng

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In sexual assault cases, forensic samples are a mixture of sperm from the perpetrator and epithelial cells from the victim. To obtain an independent short tandem repeat (STR) profile of the perpetrator, sperm cells must be separated from the mixture of cells. However, the current method used in crime laboratories, namely, differential extraction, is a time-consuming and labor-intensive process. To achieve a rapid and automated sample pretreatment process, we fabricated a microdevice for hydrodynamic and size-based separation of sperm and epithelial cells. When cells in suspension were introduced into the device's microfluidic channels, they were forced to flow along different streamlines and into different outlets due to their different diameters. With the proposed microdevice, sperm can be separated within a short period of time (0.5 h for a 50-μl mock sample). The STR profiles of the products in the sperm outlet reservoir demonstrated that a highly purified male DNA fraction could be obtained (94.0% male fraction). This microdevice is of low-cost and can be easily integrated with other subsequent analysis units, providing great potential in the process of analyzing sexual assault evidence as well as in other areas requiring cell sorting.

Identifiers

PMID26392829
PMCPMC4560716

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.