ArticleProceedings of the National Academy of Sciences of the United States of America2015
Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- The formation and function of intercellular bridges in male germlines.Communications biology · 2026Review
- Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Partial TEX14 peptides inhibit proliferation of cancer cells and have potential as anti-tumour agents.Human cell · 2025Article
- Initiation of meiosis from human iPSCs under defined conditions through identification of regulatory factors.Science advances · 2025Article
- HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice.Cellular and molecular life sciences : CMLS · 2024Article
- Correlation between TEX14 and ADAM17 expressions in colorectal cancer tissues of elderly patients and neoplasm staging, invasion, and metastasis.World journal of clinical cases · 2024Article
- ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice.Developmental biology · 2024Article
- Risk Factors for Testicular Cancer: Environment, Genes and Infections-Is It All?Medicina (Kaunas, Lithuania) · 2023Review
- Establishment and Evaluation of Dual HDAC/BET Inhibitors as Therapeutic Options for Germ Cell Tumors and Other Urological Malignancies.Molecular cancer therapeutics · 2022Article
- Identification of genes related to sexual differentiation and sterility in embryonic gonads of Mule ducks by transcriptome analysis.Frontiers in genetics · 2022Article
- Global Proteomics Analysis of Bone Marrow: Establishing Talin-1 and Centrosomal Protein of 55 kDa as Potential Molecular Signatures for Myelodysplastic Syndromes.Frontiers in oncology · 2022Article
- Uncoupling cell division and cytokinesis during germline development in metazoans.Frontiers in cell and developmental biology · 2022Review
- Novel variants in helicase for meiosis 1 lead to male infertility due to non-obstructive azoospermia.Reproductive biology and endocrinology : RB&E · 2021Article
- Altered germline cyst formation and oogenesis in Tex14 mutant mice.Biology open · 2021Article
- Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition.Development (Cambridge, England) · 2021Article
- Genetic Landscape of Nonobstructive Azoospermia and New Perspectives for the Clinic.Journal of clinical medicine · 2020Review
- [CEP55 may be a potential therapeutic target for non-obstructive azoospermia with maturation arrest].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- New insights into the genetics of spermatogenic failure: a review of the literature.Human genetics · 2019Review
- Dynamic cytoplasmic projections connect mammalian spermatogoniaDevelopment (Cambridge, England) · 2018Article
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intercellular bridges are a conserved feature of spermatogenesis in mammalian germ cells and derive from arresting cell abscission at the final stage of cytokinesis. However, it remains to be fully understood how germ cell abscission is arrested in the presence of general cytokinesis components. The TEX14 (testis-expressed gene 14) protein is recruited to the midbody and plays a key role in the inactivation of germ cell abscission. To gain insights into the structural organization of TEX14 at the midbody, we have determined the crystal structures of the EABR [endosomal sorting complex required for transport (ESCRT) and ALIX-binding region] of CEP55 bound to the TEX14 peptide (or its chimeric peptides) and performed functional characterization of the CEP55-TEX14 interaction by multiexperiment analyses. We show that TEX14 interacts with CEP55-EABR via its AxGPPx3Y (Ala793, Gly795, Pro796, Pro797, and Tyr801) and PP (Pro803 and Pro804) sequences, which together form the AxGPPx3YxPP motif. TEX14 competitively binds to CEP55-EABR to prevent the recruitment of ALIX, which is a component of the ESCRT machinery with the AxGPPx3Y motif. We also demonstrate that a high affinity and a low dissociation rate of TEX14 to CEP55, and an increase in the local concentration of TEX14, cooperatively prevent ALIX from recruiting ESCRT complexes to the midbody. The action mechanism of TEX14 suggests a scheme of how to inactivate the abscission of abnormal cells, including cancer cells.
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