ArticleProceedings of the National Academy of Sciences of the United States of America2015
Urine excretion strategy for stem cell-generated embryonic kidneys.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 74 citations in OpenAlex.
- Functional urine production and glomerular maturation from embryonic metanephros transplanted at the renal hilum in rats.Renal failure · 2026Article
- Protocol for In Utero Fetal-to-Fetal Kidney Transplantation in Rats.Bio-protocol · 2026Article
- Spatially patterned kidney assembloids recapitulate progenitor self-assembly and enable high-fidelity in vivo disease modeling.Cell stem cell · 2025Article
- Recent progress in xenotransplantation and its application to pediatric kidney disease.Pediatric nephrology (Berlin, Germany) · 2025Review
- Life-supporting functional kidney replacement by integration of embryonic metanephros-bladder composite tissue transplants.Kidney international · 2025Article
- Caspase 9-induced apoptosis enables efficient fetal cell ablation and disease modeling.Nature communications · 2025Article
- Fetal-to-fetal kidney transplantation in utero.Communications biology · 2025Article
- Article
- Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine.Journal of clinical medicine · 2023Article
- Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model.Journal of clinical medicine · 2022Article
- Kidney organoids: a pioneering model for kidney diseases.Translational research : the journal of laboratory and clinical medicine · 2022Review
- Generation of chimeric kidneys using progenitor cell replacement: Oshima Award Address 2021.Clinical and experimental nephrology · 2022Review
- Physiology assays in human kidney organoids.American journal of physiology. Renal physiology · 2022Review
- Sildenafil Citrate Enhances Renal Organogenesis Following Metanephroi Allotransplantation into Non-Immunosuppressed Hosts.Journal of clinical medicine · 2022Article
- A Novel Method for Urinary Tract Reconstruction in Transplanted Embryonic Kidneys Using the Hybrid Stent: A Pig Study.Transplantation direct · 2022Article
- Potential Strategies for Kidney Regeneration With Stem Cells: An Overview.Frontiers in cell and developmental biology · 2022Review
- Renal engineering: strategies to address the problem of the ureter.Current opinion in biomedical engineering · 2021Article
- Transplantation of Vulnerable Renal Organoids by Use of a Novel Laparoscopic Device in Pigs.Transplantation direct · 2021Article
- Article
- Microenvironment derived from metanephros transplantation inhibits the progression of acute kidney injury in glycerol-induced rat models.Renal failure · 2020Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There have been several recent attempts to generate, de novo, a functional whole kidney from stem cells using the organogenic niche or blastocyst complementation methods. However, none of these attempts succeeded in constructing a urinary excretion pathway for the stem cell-generated embryonic kidney. First, we transplanted metanephroi from cloned pig fetuses into gilts; the metanephroi grew to about 3 cm and produced urine, although hydronephrosis eventually was observed because of the lack of an excretion pathway. Second, we demonstrated the construction of urine excretion pathways in rats. Rat metanephroi or metanephroi with bladders (developed from cloacas) were transplanted into host rats. Histopathologic analysis showed that tubular lumina dilation and interstitial fibrosis were reduced in kidneys developed from cloacal transplants compared with metanephroi transplantation. Then we connected the host animal's ureter to the cloacal-developed bladder, a technique we called the "stepwise peristaltic ureter" (SWPU) system. The application of the SWPU system avoided hydronephrosis and permitted the cloacas to differentiate well, with cloacal urine being excreted persistently through the recipient ureter. Finally, we demonstrated a viable preclinical application of the SWPU system in cloned pigs. The SWPU system also inhibited hydronephrosis in the pig study. To our knowledge, this is the first report showing that the SWPU system may resolve two important problems in the generation of kidneys from stem cells: construction of a urine excretion pathway and continued growth of the newly generated kidney.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.