ArticleProceedings of the National Academy of Sciences of the United States of America2015
Formation of interference-sensitive meiotic cross-overs requires sufficient DNA leading-strand elongation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Recombination-coupled DNA synthesis facilitates post-invasion steps in meiotic crossover and noncrossover formations.Nucleic acids research · 2025Article
- FromiScience · 2024Article
- Crossover interference mechanism: New lessons from plants.Frontiers in cell and developmental biology · 2023Review
- DNA polymerase epsilon binds histone H3.1-H4 and recruits MORC1 to mediate meiotic heterochromatin condensation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparison of Metabolic Profiling ofPhenomics (Cham, Switzerland) · 2021Article
- The Formation of Bivalents and the Control of Plant Meiotic Recombination.Frontiers in plant science · 2021Review
- Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Dynamic localization of SPO11-1 and conformational changes of meiotic axial elements during recombination initiation of maize meiosis.PLoS genetics · 2020Article
- Plant DNA Polymerases.International journal of molecular sciences · 2019Review
- The Largest Subunit of DNA Polymerase Delta Is Required for Normal Formation of Meiotic Type I Crossovers.Plant physiology · 2019Article
- Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis.The Plant journal : for cell and molecular biology · 2018Article
- Arabidopsis RAD51, RAD51C and XRCC3 proteins form a complex and facilitate RAD51 localization on chromosomes for meiotic recombination.PLoS genetics · 2017Article
- Function of the Plant DNA Polymerase Epsilon in Replicative Stress Sensing, a Genetic Analysis.Plant physiology · 2017Article
- Role of the Polymerase ϵ sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in Arabidopsis.Nucleic acids research · 2016Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiosis halves diploid genomes to haploid and is essential for sexual reproduction in eukaryotes. Meiotic recombination ensures physical association of homologs and their subsequent accurate segregation and results in the redistribution of genetic variations among progeny. Most organisms have two classes of cross-overs (COs): interference-sensitive (type I) and -insensitive (type II) COs. DNA synthesis is essential for meiotic recombination, but whether DNA synthesis has a role in differentiating meiotic CO pathways is unknown. Here, we show that Arabidopsis POL2A, the homolog of the yeast DNA polymerase-ε (a leading-strand DNA polymerase), is required for plant fertility and meiosis. Mutations in POL2A cause reduced fertility and meiotic defects, including abnormal chromosome association, improper chromosome segregation, and fragmentation. Observation of prophase I cell distribution suggests that pol2a mutants likely delay progression of meiotic recombination. In addition, the residual COs in pol2a have reduced CO interference, and the double mutant of pol2a with mus81, which affects type II COs, displayed more severe defects than either single mutant, indicating that POL2A functions in the type I pathway. We hypothesize that sufficient leading-strand DNA elongation promotes formation of some type I COs. Given that meiotic recombination and DNA synthesis are conserved in divergent eukaryotes, this study and our previous study suggest a novel role for DNA synthesis in the differentiation of meiotic recombination pathways.
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