ArticleProceedings of the National Academy of Sciences of the United States of America2015
Genome and transcriptome of the regeneration-competent flatworm, Macrostomum lignano.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- A protocol for sequential isolation, purification, and collection of Luticola nematophagus eggs to obtain high-quality genomic DNA.Biology open · 2026Article
- Article
- Review
- A genetic and microscopy toolkit for manipulating and monitoring regeneration in Macrostomum lignano.Cell reports · 2024Article
- Mining differentially expressed genes during paratomy in the transcriptome of the flatworm Stenostomum leucops.Scientific reports · 2024Article
- Annelid methylomes reveal ancestral developmental and aging-associated epigenetic erosion across Bilateria.Genome biology · 2024Article
- Optimized protocols for RNA interference in Macrostomum lignano.G3 (Bethesda, Md.) · 2024Article
- Sex-limited experimental evolution drives transcriptomic divergence in a hermaphrodite.Genome biology and evolution · 2024Article
- Unraveling the Unusual Subgenomic Organization in the Neopolyploid Free-Living Flatworm Macrostomum lignano.Molecular biology and evolution · 2023Article
- Functional genomics in Spiralia.Briefings in functional genomics · 2023Article
- Genome assemblies of the simultaneously hermaphroditic flatworms Macrostomum cliftonense and Macrostomum hystrix.G3 (Bethesda, Md.) · 2023Article
- The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity.Communications biology · 2023Article
- "Omics" data unveil early molecular response underlying limb regeneration in the Chinese mitten crab,Science advances · 2022Article
- Genome Assembly of the Polyclad Flatworm Prostheceraeus crozieri.Genome biology and evolution · 2022Article
- Encyclopaedia of eukaryotic DNA methylation: from patterns to mechanisms and functions.Biochemical Society transactions · 2022Article
- MicroRNAs as Indicators into the Causes and Consequences of Whole-Genome Duplication Events.Molecular biology and evolution · 2022Article
- Three new species ofZooKeys · 2022Article
- Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Faster Rates of Molecular Sequence Evolution in Reproduction-Related Genes and in Species with Hypodermic Sperm Morphologies.Molecular biology and evolution · 2021Article
- The evolution of the metazoan Toll receptor family and its expression during protostome development.BMC ecology and evolution · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive organisms for studying the evolution of pathways involved in tissue self-renewal, cell-fate specification, and regeneration. The use of these organisms as models, however, is hampered by the lack of a well-assembled and annotated genome sequences, fundamental to modern genetic and molecular studies. Here we report the genomic sequence of M. lignano and an accompanying characterization of its transcriptome. The genome structure of M. lignano is remarkably complex, with ∼75% of its sequence being comprised of simple repeats and transposon sequences. This has made high-quality assembly from Illumina reads alone impossible (N50=222 bp). We therefore generated 130× coverage by long sequencing reads from the Pacific Biosciences platform to create a substantially improved assembly with an N50 of 64 Kbp. We complemented the reference genome with an assembled and annotated transcriptome, and used both of these datasets in combination to probe gene-expression patterns during regeneration, examining pathways important to stem cell function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.