Evidence map›Paper›PMID 26392394›Full record

ArticleMedical microbiology and immunology2016

LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood.

Lulei Zhou, Ling Liu, Jinli Yang, Yuxin Li, Wencheng Bai, Na Liu, Wenlong Li, Yumin Gao, Liping Xu, Zhi Liu and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical microbiology and immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Lulei ZhouResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Ling LiuResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Jinli YangResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Yuxin LiResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Wencheng BaiResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Na LiuResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Wenlong LiResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Yumin GaoResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Liping XuResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Zhi LiuResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China.
Runlin HanResearch Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Huhhot, China. han-runlin@163.com.
Inner Mongolia Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low-density lipoprotein (LDL) binds to group A Streptococcus (GAS) through Sc11 protein, and scavenger receptor CD36 of monocyte mediates the endocytosis of native or modified LDL. Therefore, we hypothesized that LDL might be an opsonin enhancing the phagocytosis of LDL-bound GAS by monocyte. The results showed that LDL could significantly promote U937 cell to phagocytose M28 (ATCC BAA1064) and M41 (ATCC 12373, AM41)-type GAS, and the phagocytosis rates were significantly increased, compared with LDL-free group. LDL, however, did not enhance the phagocytosis of M41 (CMCC 32198, CM41) or M6 (ATCC BAA946)-type GAS since these two strains did not bind to LDL. CD36 was the major scavenger receptor mediating the uptake of LDL-bound GAS by monocyte U937 cells since anti-CD36 antibody abolished the phagocytosis of LDL-opsonized GAS but anti-CD4 antibody did not. Most of AM41-type GAS cells were killed in human blood, whereas only a few CM41-type cells were phagocytosed. Moreover, recombinant Scl1 (rScl1) derived from M41-type GAS could significantly decrease the opsonophagocytosis of AM41 but not CM41-type GAS because the rScl1 competitively blocked the binding of AM41-type GAS to LDL. Therefore, our findings suggest that LDL may be an opsonin to enhance CD36-dependent opsonic phagocytosis of GAS by monocyte.

Indexed as

Antibodies, MonoclonalBacterial ProteinsCD36 AntigensCell LineCells, CulturedCollagenHumansLipoproteins, LDLMonocytesOpsonin ProteinsPhagocytosisRecombinant ProteinsStreptococcal InfectionsStreptococcus pyogenesU937 CellsAntibodies, MonoclonalBacterial ProteinsCD36 AntigensCollagenLipoproteins, LDLOpsonin ProteinsRecombinant ProteinsScl1 protein, StreptococcusGroup A StreptococcusLow-density lipoproteinMonocyteOpsoninPhagocytosis

Identifiers

PMID26392394
PMCPMC4792331
OpenAlexW1647499699

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.