ArticleMolecular and cellular biology2015
RNA Binding Protein Ptbp2 Is Essential for Male Germ Cell Development.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 70 citations in OpenAlex.
- Loss of full-length DAZL isoform disrupts PABPC1-dependent translational regulation and meiosis.Cell death & disease · 2025Article
- TKTL1: a new candidate gene in non-obstructive azoospermia.Reproductive biomedicine online · 2025Article
- Discordant effects of maternal age on the human MII oocyte transcriptome.Molecular human reproduction · 2025Article
- Effect of Alternative Splicing Euchromatic Histone Lysine Methyltransferase 2 (Animals : an open access journal from MDPI · 2025Article
- PTBP1 mediates Sertoli cell actin cytoskeleton organization by regulating alternative splicing of actin regulators.Nucleic acids research · 2024Article
- The Intricate Functional Networks of Pre-mRNA Alternative Splicing in Mammalian Spermatogenesis.International journal of molecular sciences · 2024Review
- The role of HnrnpF/H as a driver of oligoteratozoospermia.iScience · 2024Article
- A Comparative Full-Length Transcriptome Analysis Using Oxford Nanopore Technologies (ONT) in Four Tissues of Bovine Origin.Animals : an open access journal from MDPI · 2024Article
- Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling.eLife · 2024Article
- SRSF2 is required for mRNA splicing during spermatogenesis.BMC biology · 2023Article
- Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling.bioRxiv : the preprint server for biology · 2023Article
- Identification of New Candidate Genes Related to Semen Traits in Duroc Pigs through Weighted Single-Step GWAS.Animals : an open access journal from MDPI · 2023Article
- SRSF1-mediated alternative splicing is required for spermatogenesis.International journal of biological sciences · 2023Article
- Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation.Cell death and differentiation · 2023Article
- Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d.Science China. Life sciences · 2022Article
- hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells.Nature communications · 2022Article
- Review
- Characterization of Alternative Splicing (AS) Events during Chicken (Animals : an open access journal from MDPI · 2021Article
- SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells.Frontiers in cell and developmental biology · 2021Article
- TULP2, a New RNA-Binding Protein, Is Required for Mouse Spermatid Differentiation and Male Fertility.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
RNA binding proteins (RBPs) are increasingly recognized as essential factors in tissue development and homeostasis. The polypyrimidine tract binding (PTB) protein family of RBPs are important posttranscriptional regulators of gene expression. In the nervous system, the function and importance of PTB protein 2 (Ptbp2) as a key alternative splicing regulator is well established. Ptbp2 is also abundantly expressed during spermatogenesis, but its role in this developmental program has not been explored. Additionally, the importance of alternative splicing regulation in spermatogenesis is unclear. Here, we demonstrate that Ptbp2 is essential for spermatogenesis. We also describe an improved dual fluorescence flow cytometry strategy to discriminate, quantify, and collect germ cells in different stages of development. Using this approach, in combination with traditional histological methods, we show that Ptbp2 ablation results in germ cell loss due to increased apoptosis of meiotic spermatocytes and postmeiotic arrest of spermatid differentiation. Furthermore, we show that Ptbp2 is required for alternative splicing regulation in the testis, as in brain. Strikingly, not all of the alternatively spliced RNAs examined were sensitive to Ptbp2 loss in both tissues. Collectively, the data provide evidence for an important role for alternative splicing regulation in germ cell development and a central role for Ptbp2 in this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.