ArticleMetabolic engineering2015
Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium.
Article in Metabolic engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Determination of the genome-scale metabolic network ofFrontiers in bioengineering and biotechnology · 2025Article
- State-of the-Art Constraint-Based Modeling of Microbial Metabolism: From Basics to Context-Specific Models with a Focus on Methanotrophs.Microorganisms · 2023Review
- Insights into the rapid metabolism of Geobacillus sp. LC300: unraveling metabolic requirements and optimal growth conditions.Extremophiles : life under extreme conditions · 2023Article
- The metabolic rate of the biosphere and its components.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genome-scale reconstruction and metabolic modelling of the fast-growing thermophileMetabolic engineering communications · 2022Article
- Medium development and production of carotenoids and exopolysaccharides by the extremophile Rhodothermus marinus DSM16675 in glucose-based defined media.Microbial cell factories · 2022Article
- New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041.Applied and environmental microbiology · 2022Article
- Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant.Scientific reports · 2021Article
- Genome-scale reconstruction of Paenarthrobacter aurescens TC1 metabolic model towards the study of atrazine bioremediation.Scientific reports · 2020Article
- A Genome-Scale Metabolic Model of 2,3-Butanediol Production by Thermophilic BacteriaMicroorganisms · 2020Article
- Cultivation technology development of Rhodothermus marinus DSM 16675.Extremophiles : life under extreme conditions · 2019Article
- How adaptive evolution reshapes metabolism to improve fitness: recent advances and future outlook.Current opinion in chemical engineering · 2018Article
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
- Predicting Dynamic Metabolic Demands in the Photosynthetic EukaryotePlant physiology · 2018Article
- Cell-Free Protein Synthesis From Fast-GrowingFrontiers in microbiology · 2018Article
- A thermostable Cas9 with increased lifetime in human plasma.Nature communications · 2017Article
- Fast growth phenotype of E. coli K-12 from adaptive laboratory evolution does not require intracellular flux rewiring.Metabolic engineering · 2017Article
- Metabolism of the fast-growing bacterium Vibrio natriegens elucidated byMetabolic engineering · 2017Article
- Article
- Co-utilization of glucose and xylose by evolved Thermus thermophilus LC113 strain elucidated by (13)C metabolic flux analysis and whole genome sequencing.Metabolic engineering · 2016Article
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4 authors.
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Abstract
We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h(-1) on glucose and 1.52 h(-1) on xylose (doubling time less than 30 min). The corresponding specific glucose and xylose utilization rates are 5.55 g/g/h and 5.24 g/g/h, respectively. As such, Geobacillus LC300 grows 3-times faster than E. coli on glucose and xylose, and has a specific xylose utilization rate that is 3-times higher than the best metabolically engineered organism to date. To gain more insight into the metabolism of Geobacillus LC300 its genome was sequenced using PacBio's RS II single-molecule real-time (SMRT) sequencing platform and annotated using the RAST server. Based on the genome annotation and the measured biomass composition a core metabolic network model was constructed. To further demonstrate the biotechnological potential of this organism, Geobacillus LC300 was grown to high cell-densities in a fed-batch culture, where cells maintained a high xylose utilization rate under low dissolved oxygen concentrations. All of these characteristics make Geobacillus LC300 an attractive host for future metabolic engineering and biotechnology applications.
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