ArticleNature chemistry2015
Structure of a PEGylated protein reveals a highly porous double-helical assembly.
Article in Nature chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
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- Self-Assembled Materials Based on Fully Aromatic Peptides: The Impact of Tryptophan, Tyrosine, and Dopa Residues.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Minimal Coarse-Grained Model for Immunoglobulin G: Diffusion and Binding under Crowding.The journal of physical chemistry. B · 2023Article
- Atomic-Scale View of Protein-PEG Interactions that Redirect the Thermal Unfolding Pathway of PEGylated Human Galectin-3.Angewandte Chemie (International ed. in English) · 2022Article
- Moving Protein PEGylation from an Art to a Data Science.Bioconjugate chemistry · 2022Article
- PEGylation of the antimicrobial peptide LyeTx I-b maintains structure-related biological properties and improves selectivity.Frontiers in molecular biosciences · 2022Article
- Protein interface redesign facilitates the transformation of nanocage building blocks to 1D and 2D nanomaterials.Nature communications · 2021Article
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- DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilylNature communications · 2020Article
- Mapping protein-polymer conformations in bioconjugates with atomic precision.Chemical science · 2020Article
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- Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques.Biomolecules · 2019Review
- Construction of supramolecular nanotubes from protein crystals.Chemical science · 2019Article
- Complexation of chiral amines by resorcin[4]arene sulfonic acids in polar media - circular dichroism and diffusion studies of chirality transfer and solvent dependence.Beilstein journal of organic chemistry · 2019Article
- From Synthesis to Characterization of Site-Selective PEGylated Proteins.Frontiers in pharmacology · 2019Review
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- Intrusion of polyethylene glycol into solid-state nanopores.RSC advances · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model β-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies.
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