ArticleNature chemistry2015
Latent analysis of unmodified biomolecules and their complexes in solution with attomole detection sensitivity.
Article in Nature chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed.
- Microfluidics for protein interaction studies: current methods, challenges, and future perspectives.European biophysics journal : EBJ · 2026Review
- Microfluidic diffusional sizing in bioanalysis and biosensing.Lab on a chip · 2026Review
- Determining the Hydrodynamic Size of Lipid Nanoparticles by Fluorescent Universal Lipid Labeling for Microfluidic Diffusional Sizing (FULL-MDS).Methods in molecular biology (Clifton, N.J.) · 2026Article
- On the self-assembly of αB-crystallin.Soft matter · 2025Article
- Immunodiagnostic plasma amino acid residue biomarkers detect cancer early and predict treatment response.Nature communications · 2025Article
- Single-molecule digital sizing of proteins in solution.Nature communications · 2024Article
- Microfluidic Diffusional Sizing (MDS) Measurements of Secretory Neutralizing Antibody Affinity Against SARS-CoV-2.Annals of biomedical engineering · 2024Article
- Ganglioside Micelles Affect Amyloid β Aggregation by Coassembly.ACS chemical neuroscience · 2023Article
- On the utility of microfluidic systems to study protein interactions: advantages, challenges, and applications.European biophysics journal : EBJ · 2023Review
- Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling.STAR protocols · 2023Article
- Single-Molecule Sizing through Nanocavity Confinement.Nano letters · 2023Article
- Widespread amyloidogenicity potential of multiple myeloma patient-derived immunoglobulin light chains.BMC biology · 2023Article
- On the micelle formation of DNAJB6b.QRB discovery · 2023Article
- π-Clamp-Mediated Homo- and Heterodimerization of Single-Domain Antibodies via Site-Specific Homobifunctional Conjugation.Journal of the American Chemical Society · 2022Article
- Accelerating Reaction Rates of Biomolecules by Using Shear Stress in Artificial Capillary Systems.Journal of the American Chemical Society · 2021Article
- The binding of the small heat-shock protein αB-crystallin to fibrils of α-synuclein is driven by entropic forces.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Machine learning-aided protein identification from multidimensional signatures.Lab on a chip · 2021Article
- Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases.Nature reviews. Chemistry · 2021Review
- Rapid highly sensitive general protein quantification through on-chip chemiluminescence.Biomicrofluidics · 2021Article
- Kinetic fingerprints differentiate the mechanisms of action of anti-Aβ antibodies.Nature structural & molecular biology · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The study of biomolecular interactions is central to an understanding of function, malfunction and therapeutic modulation of biological systems, yet often involves a compromise between sensitivity and accuracy. Many conventional analytical steps and the procedures required to facilitate sensitive detection, such as the incorporation of chemical labels, are prone to perturb the complexes under observation. Here we present a 'latent' analysis approach that uses chemical and microfluidic tools to reveal, through highly sensitive detection of a labelled system, the behaviour of the physiologically relevant unlabelled system. We implement this strategy in a native microfluidic diffusional sizing platform, allowing us to achieve detection sensitivity at the attomole level, determine the hydrodynamic radii of biomolecules that vary by over three orders of magnitude in molecular weight, and study heterogeneous mixtures. We illustrate these key advantages by characterizing a complex of an antibody domain in the solution phase and under physiologically relevant conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.