ArticleScientific reports2015
Fast and Precise 3D Fluorophore Localization based on Gradient Fitting.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Particle Localization Using Local Gradients and Its Application to Nanometer Stabilization of a Microscope.ACS nano · 2022Article
- Three-dimensional imaging of biological cells using surface plasmon coupled emission.Journal of biomedical optics · 2022Article
- Robust emitter localization with enhanced harmonic analysis.Optics letters · 2021Article
- Enhanced super-resolution microscopy by extreme value based emitter recovery.Scientific reports · 2021Article
- Embedded nanometer position tracking based on enhanced phasor analysis.Optics letters · 2021Article
- Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.Current protocols in cytometry · 2020Article
- Super-resolution localization microscopy: Toward high throughput, high quality, and low cost.APL photonics · 2020Article
- Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis.Nature communications · 2020Article
- WindSTORM: Robust online image processing for high-throughput nanoscopy.Science advances · 2019Article
- Accelerating single molecule localization microscopy through parallel processing on a high-performance computing cluster.Journal of microscopy · 2019Article
- Stochastic Optical Reconstruction Microscopy (STORM).Current protocols in cytometry · 2017Article
- SSRP1 Cooperates with PARP and XRCC1 to Facilitate Single-Strand DNA Break Repair by Chromatin Priming.Cancer research · 2017Article
- A simple and cost-effective setup for super-resolution localization microscopy.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Astigmatism imaging approach has been widely used to encode the fluorophore's 3D position in single-particle tracking and super-resolution localization microscopy. Here, we present a new high-speed localization algorithm based on gradient fitting to precisely decode the 3D subpixel position of the fluorophore. This algebraic algorithm determines the center of the fluorescent emitter by finding the position with the best-fit gradient direction distribution to the measured point spread function (PSF), and can retrieve the 3D subpixel position of the fluorophore in a single iteration. Through numerical simulation and experiments with mammalian cells, we demonstrate that our algorithm yields comparable localization precision to the traditional iterative Gaussian function fitting (GF) based method, while exhibits over two orders-of-magnitude faster execution speed. Our algorithm is a promising high-speed analyzing method for 3D particle tracking and super-resolution localization microscopy.
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Registered trials
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